Engineering of capillary-like structures in tissue constructs by electrochemical detachment of cells.
Engineering of capillary-like structures in tissue constructs by electrochemical detachment of cells.
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DOI：
10.1016/j.biomaterials.2009.11.104
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发表时间：
2010-03
期刊：
影响因子：
14
通讯作者：
Y. Seto;R. Inaba;Tomoaki Okuyama;F. Sassa;H. Suzuki;J. Fukuda
中科院分区：
文献类型：
--
作者：
Y. Seto;R. Inaba;Tomoaki Okuyama;F. Sassa;H. Suzuki;J. Fukuda
功能性厚组织的发展中的主要挑战是在整个工程化组织构建体中形成用于氧气和营养供应的血管网络。本研究描述了一种电化学方法，用于制造毛细血管样结构，在微米距离内精确对齐，其内表面覆盖有血管内皮细胞。在这种方法中，一个寡肽含有细胞粘附结构域（RGD）的中心和半胱氨酸残基在两端的设计。半胱氨酸具有通过金-硫醇盐键吸附到金表面上的硫醇基团。通过寡肽附着到金表面的细胞通过施加负电位并切割金-硫醇盐键而容易且非侵入性地分离。这种方法不仅适用于平面，而且适用于各种配置，包括圆柱形结构。通过将这种方法应用于在灌注培养装置中以空间控制的方式排列的细金棒，将人脐静脉内皮细胞（HUVEC）转移到胶原凝胶中的毛细血管结构的内表面上。在随后的灌注培养中，HUVECs生长到胶原凝胶中并形成管腔结构，从而在体外形成血管网络。
A major challenge in the development of functional thick tissues is the formation of vascular networks for oxygen and nutrient supply throughout the engineered tissue constructs. This study describes an electrochemical approach for fabrication of capillary-like structures, precisely aligned within micrometer distances, whose internal surfaces are covered with vascular endothelial cells. In this approach, an oligopeptide containing a cell adhesion domain (RGD) in the center and cysteine residues at both ends was designed. Cysteine has a thiol group that adsorbs onto a gold surface via a gold–thiolate bond. The cells attached to the gold surface via the oligopeptide were readily and noninvasively detached by applying a negative electrical potential and cleaving the gold–thiolate bond. This approach was applicable not only for a flat surface but also for various configurations, including cylindrical structures. By applying this approach to thin gold rods aligned in a spatially controlled manner in a perfusion culture device, human umbilical vein endothelial cells (HUVECs) were transferred onto the internal surface of capillary structures in collagen gel. In the subsequent perfusion culture, the HUVECs grew into the collagen gel and formed luminal structures, thereby forming vascular networks in vitro.