Selective inhibition of Alu retrotransposition by APOBEC3G
Selective inhibition of Alu retrotransposition by APOBEC3G
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DOI：
10.1016/j.gene.2006.08.032
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发表时间：
2007-04-01
期刊：
影响因子：
3.5
通讯作者：
Moran, John V.
中科院分区：
文献类型：
--
作者：
Hulme, Amy E.;Bogerd, Hal P.;Moran, John V.
非LTR反转录转座子LINE-1（L1）包含类似于17%的人类基因组，并且L1编码的蛋白质可以反式起作用以介导非自主反转录转座子的反转录转座（即，Alu和可能的SVA元件）和细胞mRNA以产生加工的假基因。在这里，我们研究了APOBEC 3G和APOBEC 3F，胞苷脱氨酶，抑制VIF缺陷的HIV-1复制，对Alu逆转录和其他L1介导的逆转录过程的影响。我们证明，APOBEC 3G选择性抑制Alu逆转录转座在OR-F I p-依赖的方式。活性胞苷脱氨酶位点不需要抑制Alu逆转录转座，并且所得整合事件缺乏G至A或C至T超突变。这些数据证明了L1和Alu逆转录转座由APOBEC 3G的差异限制，并表明Alu核糖核蛋白复合物可能是由APOBEC 3G靶向的。(c)2006 Elsevier B. V.保留所有权利。
The non-LTR retrotransposon LINE-1 (L1) comprises similar to 17% of the human genome, and the L1-encoded proteins can function in trans to mediate the retrotransposition of non-autonomous retrotransposons (i.e., Alu and probably SVA elements) and cellular mRNAs to generate processed pseudogenes. Here, we have examined the effect of APOBEC3G and APOBEC3F, cytidine deaminases that inhibit Vif-deficient HIV-1 replication, on Alu retrotransposition and other L1-mediated retrotransposition processes. We demonstrate that APOBEC3G selectively inhibits Alu retrotransposition in an OR-F I p-in dependent manner. An active cytidine deaminase site is not required for the inhibition of Alu retrotransposition and the resultant integration events lack G to A or C to T hypermutation. These data demonstrate a differential restriction of L1 and Alu retrotransposition by APOBEC3G, and suggest that the Alu ribonucleoprotein complex may be targeted by APOBEC3G. (c) 2006 Elsevier B.V. All rights reserved.