The Protective Effect of Sodium Ferulate and Oxymatrine Combination on Paraquat-induced Lung Injury
The Protective Effect of Sodium Ferulate and Oxymatrine Combination on Paraquat-induced Lung Injury
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DOI：
10.22037/ijpr.2015.1642
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发表时间：
2015-05
期刊：
影响因子：
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通讯作者：
Wei Wang;X. Pei;Mengxin Xu;Songmei Sun;Chunlei Zhang;Keying Mu;Zhifeng Liu
中科院分区：
文献类型：
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作者：
Wei Wang;X. Pei;Mengxin Xu;Songmei Sun;Chunlei Zhang;Keying Mu;Zhifeng Liu
实验结果表明，阿魏酸钠（SF）和氧化苦参碱（OMT）合用具有协同抗炎和抗氧化作用。我们推测SF和OMT联合治疗可能对百草枯诱导的急性肺损伤具有保护作用。在我们的研究中，瑞士小鼠被随机分为七组，包括对照组、百草枯（PQ）组、SF（6.2 mg/Kg/d）组、OMT（13.8 mg/Kg/d）组和SF+OMT三个组（3.1 + 6.9、6.2 + 13.8和12.3 + 27.7 mg/Kg/d）。监测死亡率和死亡时间。将Sprague-Dawley大鼠随机分为7组，包括对照组、PQ组、SF组（3.1 mg/Kg/天）、OMT组（6.9 mg/Kg/天）和3个SF+OMT组（1.6 + 3.4、3.1 + 6.9和6.2 + 13.8 mg/Kg/天）。观察肺湿/干重（W/D）比值、肺组织病理学改变、C反应蛋白（CRP）、白细胞介素6（IL-6）、核因子κB（NF-κB）、丙二醛（MDA）和超氧化物歧化酶（SOD）的变化。与PQ组相比，SF和OMT联合治疗组小鼠死亡率显著降低，死亡时间延长。SF和OMT联合治疗组大鼠肺W/D比值和肺组织病理学评分均显著降低，血清和肺组织匀浆中CRP、IL-6、NF-κB和MDA水平显著降低，血清和肺组织匀浆中SOD活性显著升高。这些结果表明SF和OMT联合应用对PQ引起的肺损伤具有明显的保护作用。其机制可能与抗炎、抗氧化作用有关。
Experimental evidence suggested that sodium ferulate (SF) and oxymatrine (OMT) combination had synergistic anti-inflammatory and antioxidant effects. We hypothesized that SF and OMT combination treatment might have protective effects on paraquat-induced acute lung injury. In our study, the Swiss mice were randomly divided into seven groups, including control, paraquat (PQ), SF (6.2 mg/Kg/day); OMT (13.8 mg/Kg/day) and three SF+OMT groups (3.1 + 6.9; 6.2 + 13.8 and 12.3 + 27.7 mg/Kg/day). The mortality and death time were monitored. Sprague-Dawley rats were randomly divided into seven groups including control, PQ, SF (3.1 mg/Kg/day); OMT (6.9 mg/Kg/day) and three SF+OMT groups (1.6 + 3.4; 3.1 + 6.9 and 6.2 + 13.8 mg/Kg/day). The lung wet/dry weight (W/D) ratio, lung histopathologic changes, C-reactive protein (CRP), interleukin-6 (IL-6), nuclear factor κB (NF-κB), malondialdehyde (MDA) and superoxidase dismutase (SOD) were analysed. Compared with PQ group, the mortality significantly decreased and the death time prolonged in SF and OMT combination treatment groups of mice. Also in SF and OMT combination treatment groups of rats, the increased lung W/D ratio and histopathological score induced by PQ injection were significantly decreased; the levels of CRP, IL-6, NF-κB and MDA in serum and lung homogenate were significantly decreased; the SOD activities in serum and lung homogenate were improved. These results suggested that SF and OMT combination had an obvious protective effect on PQ-induced lung injury. The anti-inflammatory and antioxidant effect might be involved in the mechanism.