Estradiol protects against alteration of protein kinase C(epsilon) in a binge model of ethanol dependence and withdrawal.
Estradiol protects against alteration of protein kinase C(epsilon) in a binge model of ethanol dependence and withdrawal.
复制标题
在乙醇依赖和戒断的暴饮暴食模型中，雌二醇可防止蛋白激酶 C(epsilon) 的改变。
DOI：
10.1016/j.ejphar.2005.03.038
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Simpkins,JamesWilliam
中科院分区：
文献类型：
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作者：
Jung,MariannaEunsun;Jacobs,Stephanie;Rewal,Mridula;Wilson,Andrew;Simpkins,JamesWilliam
本研究验证了一种假设，即酒精摄入和酒精戒断会以17β-雌二醇保护的方式干扰蛋白激酶C（PKC）的稳态。植入17β-雌二醇或油丸的卵巢切除大鼠通过管饲法接受乙醇（7.5%重量/体积，7天）或对照溶液。在乙醇暴露或乙醇戒断期间收集小脑，使用ATP磷酸化和免疫印迹分析来评估PKC β和总PKC的活性、蛋白水平和细胞分布。而乙醇暴露和乙醇戒断均增加膜蛋白水平和膜转位，只有乙醇戒断增强PKC β的活性。酒精戒断后总PKC的三个参数均升高，而酒精暴露后无此作用。17β-雌二醇治疗防止了PKC谱的这些变化。这些数据表明，过量的间歇性摄入乙醇，然后乙醇戒断干扰PKC的稳态和细胞分布的PKC，特别是PKC β，在雌激素的保护方式。PKC β在乙醇戒断过程中比在乙醇暴露过程中更脆弱。
This study tested the hypothesis that a binge type of ethanol intake and ethanol withdrawal disturbs protein kinase C (PKC) homeostasis in a manner protected by 17β-estradiol. Ovariectomized rats implanted with 17β-estradiol or oil pellets received ethanol (7.5% weight/volume, 7 days) or control solution by a gavage method. The cerebelli were collected during ethanol exposure or ethanol withdrawal to assess the activity, protein levels, and cellular distribution of PKCɛ and total PKC, using an ATP phosphorylation and immunoblot assays. While both ethanol exposure and ethanol withdrawal increased membrane protein levels and membrane translocation, only ethanol withdrawal enhanced activity of PKCɛ. Ethanol withdrawal not ethanol exposure increased the three parameters of total PKC. 17β-Estradiol treatment prevented these changes in PKC profiles. These data suggest that an excessive episodic intake of ethanol followed by ethanol withdrawal disturbs PKC homeostasis and cellular distribution of PKC, in particular PKCɛ, in a manner that is protected by estrogen. PKCɛ appears more vulnerable during ethanol withdrawal than during ethanol exposure.