Maslinic acid activates mTORC1 and human TGR5 and induces skeletal muscle hypertrophy
Maslinic acid activates mTORC1 and human TGR5 and induces skeletal muscle hypertrophy
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DOI：
10.1093/bbb/zbab151
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发表时间：
2021-08-30
影响因子：
1.6
通讯作者：
Sato, Ryuichiro
中科院分区：
文献类型：
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作者：
Murata, Shotaro;Sasaki, Takashi;Sato, Ryuichiro
山楂酸是一种天然存在于30多种植物（包括橄榄）中的五环三萜，据报道可以增加人体肌肉质量和肌肉力量;然而，其潜在的分子机制仍然未知。给C57 BL/6 J小鼠喂食标准饮食或补充0.27%山楂酸4周，并测量其骨骼肌质量。食用山楂酸的小鼠显示腓肠肌和比目鱼肌质量显著增加。用雷帕霉素复合物1（mTORC 1）或蛋白激酶B（Akt）抑制剂处理培养的小鼠-C2 C12骨骼肌细胞，并定量蛋白质合成。山楂酸通过mTORC 1激活促进蛋白质合成，而不依赖于Akt。此外，山楂酸激活人武田G蛋白偶联受体5（TGR 5）比小鼠TGR 5更强，增加了几个基因的表达与肌肉肥大。山楂酸激活mTORC 1和人TGR 5，这意味着它通过这些作用对人肌肉肥大的贡献。
Maslinic acid, a naturally occurring pentacyclic triterpene in more than 30 plants (including olives), reportedly increases human muscle mass and muscle strength; however, the underlying molecular mechanism remains unknown. C57BL/6J mice were fed a standard diet or supplemented with 0.27% maslinic acid for 4 weeks, and their skeletal muscle mass was measured. Mice that consumed maslinic acid displayed significant increases in gastrocnemius and soleus muscle mass. Cultured mouse-C2C12 skeletal muscle cells were treated with mammalian target of rapamycin complex 1 (mTORC1) or protein kinase b (Akt) inhibitor, and protein synthesis was quantified. Maslinic acid accelerated protein synthesis via mTORC1 activation independent of Akt. Furthermore, maslinic acid activated human Takeda G protein-coupled receptor 5 (TGR5) more strongly than mouse TGR5, augmenting the expression of several genes related to muscular hypertrophy. Maslinic acid activated mTORC1 and human TGR5, implying its contribution to human muscular hypertrophy through these effects.