Detecting and tracking local changes in the tibiae of individual rats: a novel method to analyse longitudinal in vivo micro-CT data
Detecting and tracking local changes in the tibiae of individual rats: a novel method to analyse longitudinal in vivo micro-CT data
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DOI：
10.1016/j.bone.2003.08.012
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发表时间：
2004-01-01
期刊：
影响因子：
4.1
通讯作者：
Weinans, H
中科院分区：
文献类型：
--
作者：
Waarsing, JH;Day, JS;Weinans, H
在这项研究中，我们提出了一种新的方法，准确地评估纵向显微CT研究的基础上，图像配准，在体内显微CT扫描的分析。我们测试了是否可以在个体动物中检测和跟踪骨骼结构的详细变化。开发了一种原型体内微型CT扫描仪（Skyscan 1076），在该扫描仪中对躺在床上的处于气体麻醉下的大鼠进行了扫描。本研究使用了三只雌性Wistar大鼠：一只假手术大鼠、一只卵巢切除（OVX）大鼠和一只用作再现性对照的大鼠。在1天内扫描再现性对照大鼠两次。其他动物在第0周、手术前、手术后第4周和第14周进行扫描。通过使用使用互信息进行最佳配准的算法将在不同时间点获得的两个数据集重叠，检测随时间推移的结构变化。重复性测试显示，骨体积测量误差小于3%，骨小梁厚度测量误差小于0.5%。假手术大鼠的总骨体积没有变化，但可以检测到一些小骨小梁变薄和最终丢失，这可能与动物的年龄有关。OVX大鼠骨小梁体积丢失较多，尤其是在干骺端（第4周为60%，第14周为75%）。剩余的骨小梁厚度缓慢增加。随时间的不同扫描显示新的小梁结构的形成。此外，在前4周后可以检测到生长板处的小纵向生长。此外，OVX大鼠表现出广泛的建模在近端骨内膜外侧cortics.We已经显示了一种新的方法，可以检测和跟踪的变化，在局部骨结构和个别小梁的时间，在一个单独的活动物。该方法能够进行纵向体内显微CT研究，并有可能极大地促进大鼠或小鼠的药理学干预和转基因模型的实验研究。(C)2003年爱思唯尔公司All rights reserved.
In this study we present the analysis of in vivo micro-CT scans using a new method based on image registration that accurately evaluates longitudinal micro-CT studies. We tested if detailed changes in the bone architecture could be detected and tracked in individual animals.A prototype in vivo micro-CT scanner (Skyscan 1076) was developed in which tibiae of rats that are lying on a bed under gas anaesthesia were scanned. For this study, three female Wistar rats were used: a sham-operated rat, an ovariectomised (OVX) rat and one rat that served as a reproducibility control. The reproducibility control rat was scanned twice in I day. The other animals were scanned at week 0, just before surgery, at week 4 and at week 14 after surgery. Architectural changes over time were detected by overlaying two data sets made at different time points using an algorithm that uses mutual information for optimal registration. The scans were segmented into binary data sets using a local thresholding algorithm.The reproducibility test showed small errors of less than 3% in bone volume measurements and errors less than 0.5% in measurements of trabecular thickness.The sham-operated rat showed no changes in total bone volume, though thinning and eventual loss of some small trabeculae could be detected, which could be related to the age of the animal. The OVX rat lost much trabecular bone volume, especially in the metaphysis (60% at week 4, 75% at week 14). The remaining trabeculae slowly increased in thickness. Following the different scans in time showed the forming of new trabecular structures. Additionally, small longitudinal growth at the growth plate could be detected after the first 4 weeks. Further, the OVX rat showed extensive modelling at the proximal endosteal lateral cortex.We have shown a new method that can detect and track changes in the local bone architecture and individual trabeculae in time, in an individual living animal. This method enables longitudinal in vivo micro-CT studies and has the potential to greatly contribute to experimental rat or mouse studies on pharmacological intervention and transgenic models.(C) 2003 Elsevier Inc. All rights reserved.