Shape changes in human erythrocytes induced by replacement of the native phosphatidylcholine with species containing various fatty acids
Shape changes in human erythrocytes induced by replacement of the native phosphatidylcholine with species containing various fatty acids
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用含有各种脂肪酸的物质替代天然磷脂酰胆碱引起的人红细胞形状变化
DOI：
--
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发表时间：
1984
影响因子：
7.8
通讯作者：
L. M. Deenen
中科院分区：
文献类型：
--
作者：
F. Kuypers;B. Roelofsen;W. Berendsen;J. O. D. Kamp;L. M. Deenen
来自牛肝的磷脂酰胆碱特异性转移蛋白已被用于替代天然磷脂酰胆碱（PC）分子从完整的人红细胞通过多种不同的磷脂酰胆碱种类的脂肪酸组成。这些替代既没有改变膜的总磷脂含量，也没有改变这个部分的各种磷脂类的组成。用1-棕榈酰PC、2-油基PC、1-棕榈酰PC、2-亚油基PC、卵PC或从大鼠肝微粒体中分离的PC代替天然PC时，红细胞形态未发生改变。与不饱和物质1,2-二豆芽糖酰PC、1,2-二棕榈酰PC和1,2-二硬脂酰PC的替代导致了棘细胞的形成，并且在更高水平的替代下，在球形棘细胞中形成。用1-棕榈酰、2-花生四烯酰基PC替代后也观察到棘细胞样红细胞，而用含有1,2-二油基PC和1,2-二油基PC等两种不饱和脂肪酸的PC替代后形成了口细胞。观察结果表明，含有一种饱和脂肪酸和一种单不饱和脂肪酸或双不饱和脂肪酸的PC物种最能稳定红细胞膜结构和人红细胞的整体盘状细胞形状，并且细胞只能容忍其PC物种组成的有限变化。
Phosphatidylcholine-specific transfer protein from beef liver has been used to replace native phosphatidylcholine (PC) molecules from intact human erythrocytes by a variety of PC species differing in fatty acid composition. These replacements changed neither the total phospholipid content of the membrane, nor the composition of this fraction in terms of the various phospholipid classes. The morphology of the erythrocyte was not modified when native PC was replaced by 1-palmitoyl,2-oleoyl PC, 1-palmitoyl,2-linoleoyl PC, egg PC, or PC isolated from rat liver microsomes. Replacement with the disaturated species 1,2-dimyristoyl PC, 1,2-dipalmitoyl PC, and 1,2-distearoyl PC resulted in the formation of echinocytes and, at higher levels of replacement, in spheroechinocytes. Echinocyte-like erythrocytes were also observed after replacement with 1-palmitoyl,2-arachidonoyl PC, whereas stomatocytes were formed upon replacement with PC species containing two unsaturated fatty acids, e.g., 1,2-dioleoyl PC and 1,2-dilinoleoyl PC. The observations show that the erythrocyte membrane structure and the overall discoid cell shape of the human erythrocyte are optimally stabilized by PC species that contain one saturated and one mono- or diunsaturated fatty acid, and that the cell tolerates only limited variations in the species composition of its PC.
DOI：
10.1016/s0021-9258(19)70566-3
发表时间：
1980-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Y. Lange;H. B. Cutler;T. Steck
通讯作者：
Y. Lange;H. B. Cutler;T. Steck
胆固醇和溶血磷脂酰胆碱在确定红细胞形状中的相互作用。
影响因子：
6.5
作者：
Lange,Y;Slayton,JM
通讯作者：
Slayton,JM
DOI：
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发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lange,Y;Dolde,J;Steck,TL
通讯作者：
Steck,TL