SPECIFIC BINDING OF HOST-CELL PROTEINS TO THE 3'-TERMINAL STEM-LOOP STRUCTURE OF RUBELLA-VIRUS NEGATIVE-STRAND RNA
SPECIFIC BINDING OF HOST-CELL PROTEINS TO THE 3'-TERMINAL STEM-LOOP STRUCTURE OF RUBELLA-VIRUS NEGATIVE-STRAND RNA
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DOI：
10.1128/jvi.65.11.5961-5967.1991
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发表时间：
1991-11-01
影响因子：
5.4
通讯作者：
LIU, TY
中科院分区：
文献类型：
--
作者：
NAKHASI, HL;CAO, XQ;LIU, TY
在风疹病毒基因组RNA的5'端，有一些序列可以形成潜在稳定的茎环（SL）结构。 风疹病毒RNA正链5'末端SL结构的互补负链等效物[5'(+)SL结构]被认为是启动正链合成的启动子。 我们筛选了 5' (+) SL 结构（64 个核苷酸）的负链等效物以及负链 RNA 的邻近区域，以确定它们与宿主细胞蛋白质结合的能力。 通过紫外线诱导的共价交联，观察到相对分子质量分别为 97、79 和 56 kDa 的三种胞浆蛋白与 64 核苷酸长的潜在 SL 结构的特异性结合。 感染风疹病毒后，细胞中 97-kDa 蛋白的结合显着增加。 通过删除两个潜在环之一中的序列来改变 SL 结构，从而消除了结合相互作用。 56-kDa 蛋白似乎还与源自正链 RNA 3' 端的 SL 特异性结合。 风疹病毒负链RNA的3'末端结构与甲病毒（例如辛德比斯病毒和东部马脑炎病毒）中的相似结构具有相同的蛋白质结合活性。 讨论了宿主蛋白在风疹病毒和甲病毒复制中的可能作用。
At the 5' end of the rubella virus genomic RNA, there are sequences that can form a potentially stable stem-loop (SL) structure. The complementary negative-strand equivalent of the 5'-end SL structure of positive-strand rubella virus RNA [5' (+) SL structure] is thought to serve as a promoter for the initiation of positive-strand synthesis. We screened the negative-strand equivalent of the 5' (+) SL structure (64 nucleotides) and the adjacent region of the negative-strand RNA for their ability to bind to host cell proteins. Specific binding to the 64-nucleotide-long potential SL structure of three cytosolic proteins with relative molecular masses of 97, 79, and 56 kDa was observed by UV-induced covalent cross-linking. There was a significant increase in the binding of the 97-kDa protein from cells upon infection with rubella virus. Altering the SL structure by deleting sequences in either one of the two potential loops abolished the binding interaction. The 56-kDa protein also appeared to bind specifically to an SL derived from the 3' end of positive-strand RNA. The 3'-terminal structure of rubella virus negative-strand RNA shared the same protein-binding activity with similar structures in alphaviruses, such as Sindbis virus and eastern equine encephalitis virus. A possible role for the host proteins in the replication of rubella virus and alphaviruses is discussed.