Overexpression of Rice Glutaredoxin OsGrx_C7 and OsGrx_C2.1 Reduces Intracellular Arsenic Accumulation and Increases Tolerance in Arabidopsis thaliana.
Overexpression of Rice Glutaredoxin OsGrx_C7 and OsGrx_C2.1 Reduces Intracellular Arsenic Accumulation and Increases Tolerance in Arabidopsis thaliana.
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DOI：
10.3389/fpls.2016.00740
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发表时间：
2016
影响因子：
5.6
通讯作者：
Chakrabarty D
中科院分区：
文献类型：
--
作者：
Verma PK;Verma S;Pande V;Mallick S;Deo Tripathi R;Dhankher OP;Chakrabarty D
谷氧还蛋白(Glutaredoxins，Grxs)是一类小分子多功能蛋白质家族，参与多种细胞功能，包括氧化还原调节和氧化应激保护。尽管在植物基因组中存在大量的GRX基因(水稻中有48个GRX)，但大多数GRX基因的生物学功能和生理作用尚不清楚。本文报道了两个对砷敏感的水稻GRX家族蛋白OsGrx_C7和OsGrx_c2.1的功能特性。在转基因拟南芥中过表达OsGrx_C7和OsGrx_c2.1，从萌发、根生长和全株生长等方面反映了它们对砷的耐受性。此外，在AsIII和ASV胁迫下，拟南芥种子和茎组织中OsGrxs的转基因表达显著低于野生型植株。因此，OsGrx_C7和OsGrx_c2.1似乎是植物AS胁迫反应的重要决定因素。转OsGrx_C7和OsGrx_c2.1基因维持了细胞内的GSH池，并通过排泄或通过改变A.thaliana AtNIPs的转录本来减少AsIII的摄取。总之，OsGrx_C7和OsGrx_c2.1可能代表了参与AS胁迫反应的GRX家族蛋白，可能有助于更好地了解As诱导的胁迫途径，并为提高作物的抗逆性和降低As含量提供更好的策略。
Glutaredoxins (Grxs) are a family of small multifunctional proteins involved in various cellular functions, including redox regulation and protection under oxidative stress. Despite the high number of Grx genes in plant genomes (48 Grxs in rice), the biological functions and physiological roles of most of them remain unknown. Here, the functional characterization of the two arsenic-responsive rice Grx family proteins, OsGrx_C7 and OsGrx_C2.1 are reported. Over-expression of OsGrx_C7 and OsGrx_C2.1 in transgenic Arabidopsis thaliana conferred arsenic (As) tolerance as reflected by germination, root growth assay, and whole plant growth. Also, the transgenic expression of OsGrxs displayed significantly reduced As accumulation in A. thaliana seeds and shoot tissues compared to WT plants during both AsIII and AsV stress. Thus, OsGrx_C7 and OsGrx_C2.1 seem to be an important determinant of As-stress response in plants. OsGrx_C7 and OsGrx_C2.1 transgenic showed to maintain intracellular GSH pool and involved in lowering AsIII accumulation either by extrusion or reducing uptake by altering the transcript of A. thaliana AtNIPs. Overall, OsGrx_C7 and OsGrx_C2.1 may represent a Grx family protein involved in As stress response and may allow a better understanding of the As induced stress pathways and the design of strategies for the improvement of stress tolerance as well as decreased As content in crops.