Modulation of protein behavior through light responses of TiO2 nanodots films.
Modulation of protein behavior through light responses of TiO2 nanodots films.
复制标题
通过 TiO2 纳米点薄膜的光响应调节蛋白质行为
DOI：
10.1038/srep13354
复制
发表时间：
2015-08-26
影响因子：
4.6
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Cheng K;Hong Y;Yu M;Lin J;Weng W;Wang H
在这项工作中，蛋白质分子吸附在二氧化钛纳米点膜上的行为是通过纳米点光响应来调节的。首次通过相分离诱导自组装法制备了二氧化钛纳米点膜。然后，牛血清白蛋白(BSA)被吸附在二氧化钛纳米点膜上，并在365 nm的紫外光照射下。研究发现，表面结合的BSA分子的构象随着紫外光的照射而发生变化。此外，结合在覆盖层中的表面结合分子的BSA分子被释放。究其原因，主要是由于二氧化钛纳米点吸收了紫外光，导致纳米点表面羟基增多。这种增加进一步导致了表面结合的BSA分子中-NH3基团的吸引增强。这不仅改变了表面结合的BSA分子的构象，而且削弱了表面结合的分子与覆盖层中其他BSA分子之间的共轭作用。最终，BSA分子的覆盖层被释放。人们认为，这种通过光响应引起的蛋白质构象变化和释放行为对于理解纳米结构的生物医学性能是至关重要的。此外，它还可以广泛应用于材料-蛋白质相互作用的剪裁。
In this work, the behavior of protein molecules adsorbed on TiO2 nanodots films are modulated through the light responses of the nanodots. TiO2 nanodots films are first prepared through phase separation induced self assembly. Then, bovine serum albumin (BSA) is adsorbed on TiO2 nanodots films and exposed to ultraviolet (365 nm) illumination. It is found the conformation of surface-bound BSA molecules changes with ultraviolet illumination. Moreover, the BSA molecules conjugate to the surface-bound molecules, which are in the overlayer, are released. The reason is ascribed to that TiO2 nanodots absorb ultraviolet and result in the increase of surface hydroxyl groups on nanodots. Such increase further leads to intensified attraction of -NH3 groups in the surface-bound BSA molecules. That not only changes the conformation of the surface-bound BSA molecules, but also weaken the conjugation between surface-bound molecules and other BSA molecules in the overlayer. Eventually, the overlayer of BSA molecules is released. It is believed that such protein conformation variation and release behavior induced through light responses of TiO2 nanodots are crucial in understanding the biomedical performance of TiO2 nanostructures. Also, it could be widely utilized in tailoring of the materials-protein interactions.