Phylogenetic and metabolic diversity of bacteria degrading aromatic compounds under denitrifying conditions, and description of Thauera phenylacetica sp nov., Thauera aminoaromatica sp nov., and Azoarcus buckelii sp nov.
Phylogenetic and metabolic diversity of bacteria degrading aromatic compounds under denitrifying conditions, and description of Thauera phenylacetica sp nov., Thauera aminoaromatica sp nov., and Azoarcus buckelii sp nov.
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DOI：
10.1007/s00203-002-0422-6
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发表时间：
2002-07-01
影响因子：
2.8
通讯作者：
Fuchs, G
中科院分区：
文献类型：
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作者：
Mechichi, T;Stackebrandt, E;Fuchs, G
d 通过 16S rRNA 基因测序、确定生理和形态特征以及 DNA-DNA 杂交，对从不同单环芳香族基质上的各种含氧和缺氧生境中分离的六株反硝化细菌进行了表征。根据这些标准，菌株S100、S、P和LG356被鉴定为Thaueraaromatica的成员。菌株 B5-1 和 N5-2 暂时属于 Azoarcus tolulyticus。菌株 B4P 和 S2 彼此之间以及与其他描述的 Thauera 物种之间仅存在远亲关系。这两个菌株被提议作为两个新种Thauera phenaceta sp.的模式菌株。十一月和 Thaueraaminoaromatica sp。十一月，分别。通过16S rRNA基因分析，菌株U120与典型菌株Azoarcus evansii和Azoarcus anaerobius高度相关，而相应的DNA-DNA重联值表明基因组相关性较低。基于较低的 DNA 相似性值和独特的生理特性，菌株 U120 被提议作为新物种 Azoarcus buckelii sp 的模式菌株。十一月几乎所有新分离株都是用不同的底物获得的。分离物的高度变化的底物光谱表明，通过使用更大范围的芳香族底物进行富集和分离，在不同的生境中将会发现更高多样性的降解芳香族化合物的反硝化细菌。
dSix strains of denitrifying bacteria isolated from various oxic and anoxic habitats on different monocyclic aromatic substrates were characterized by sequencing 16S rRNA genes, determining physiological and morphological traits, and DNA-DNA hybridization. According to these criteria, strains S100, S,P and LG356 were identified as members of Thauera aromatica. Strains B5-1 and N5-2 were tentatively affiliated to the species Azoarcus tolulyticus. Strains B4P and S2 were only distantly related to each other and to other described Thauera species. These two strains are proposed as the type strains of two new species, Thauera phenylacetica sp. nov. and Thauera aminoaromatica sp. nov., respectively. By 16S rRNA gene analysis, strain U120 was highly related to the type strains of Azoarcus evansii and Azoarcus anaerobius, whereas corresponding DNA-DNA reassociation values indicated only a low degree of genomic relatedness. Based upon a low DNA similarity value and the presence of distinguishing physiological properties, strain U120 is proposed as the type strain of a new species, Azoarcus buckelii sp. nov. Almost all of the new isolates were obtained with different substrates. The highly varied substrate spectra of the isolates indicates that an even higher diversity of denitrifying bacteria degrading aromatic compounds would be discovered in the different habitats by using a larger spectrum of aromatic substrates for enrichment and isolation.