CHANGES IN THE EXPRESSION OF ALPHA-FODRIN DURING EMBRYONIC-DEVELOPMENT OF XENOPUS-LAEVIS
CHANGES IN THE EXPRESSION OF ALPHA-FODRIN DURING EMBRYONIC-DEVELOPMENT OF XENOPUS-LAEVIS
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DOI：
10.1083/jcb.105.2.843
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发表时间：
1987-08-01
影响因子：
7.8
通讯作者：
MOON, RT
中科院分区：
文献类型：
--
作者：
GIEBELHAUS, DH;ZELUS, BD;MOON, RT
胞膜蛋白（非红细胞血影蛋白）及其相关蛋白质先前已涉及在分化细胞中建立专门的膜细胞骨架结构域。使用单特异性针对α- fodrin的亚基，我们证明了α-胞衬蛋白存在于非洲爪蟾的卵母细胞和成体组织中。α-β-D-半胱氨酸的从头合成的分析在胚胎发育期间的胞衬蛋白揭示了α-胞衬蛋白在卵母细胞中合成，但不在早期发育期间合成。为了研究α-半乳糖苷酶的控制水平，为了表达胞衬蛋白，我们分离了卵母细胞α-胞衬蛋白的两个cDNA克隆。福德林。卵母细胞cDNA克隆被鉴定为α-IFN α的编码部分。基于DNA序列分析和cDNA的预测氨基酸序列与人红细胞α-胞衬蛋白的已知序列的比较，血影蛋白非洲爪蟾胞衬蛋白cDNA在RNA印迹上与约9 kb的转录物杂交，在基因组DNA印迹上可能与单个基因类型杂交。RNA印迹分析和S1核酸酶保护测定均使用非洲爪蟾α-胞衬蛋白cDNA证实，观察到的α-胞衬蛋白从头合成的下降，胞衬蛋白多肽受α-胞衬蛋白丰度的显著降低控制。受精后的胞衬蛋白转录本。相反，肌动蛋白转录水平在此期间不下降。由于α-γ的稳态水平胞衬蛋白转录物在发育的神经胚阶段上升，这些结果表明α-胞衬蛋白的合成胞衬蛋白多肽在非洲爪蟾胚胎发育过程中是受调控的，而不是组成型的，控制的主要水平是mRNA的稳态丰度。
Fodrin (nonerythroid spectrin) and its associated proteins have been previously implicated in the establishment of specialized membrane-cytoskeletal domains in differentiating cells. Using antiserum which is monospecific for the .alpha.-subunit of fodrin, we demonstrate that .alpha.-fodrin is present in oocytes and adult tissues of Xenopus laevis. Analyses of the de novo synthesis of .alpha.-fodrin during embryonic development reveal that .alpha.-fodrin is synthesized in oocytes, but not during early development. To investigate the level of control of .alpha.-fodrin expression, we isolated two cDNA clones for oocyte .alpha.-fodrin. The oocyte cDNA clones were identified as encoding portions of .alpha.-fodrin based on DNA sequence analysis and on the comparison of the predicted amino acid sequence of the cDNAs with the known sequence of human erythrocyte .alpha.-spectrin. The Xenopus .alpha.-fodrin cDNAs hybridize to a transcript of .apprx.9 kb on RNA blots, and probably to single gene type on genomic DNA blots. Both RNA blot analyses and S1 nuclease protection assays with the Xenopus .alpha.-fodrin cDNAs demonstrate that the observed decline in the de novo synthesis of .alpha.-fodrin polypeptides is controlled by a dramatic decrease in the abundance of .alpha.-fodrin transcripts after fertilization. In contrast, levels of actin transcripts do not decrease during this period. Inasmuch as steady-state levels of .alpha.-fodrin transcripts rise by the neurula stage of development, these results suggest that the synthesis of .alpha.-fodrin polypeptides during embryonic development of Xenopus is regulated, rather than constitutive, and that the primary level of control is the steady-state abundance of mRNA.