Endocrine and inflammatory factors and endometriosis-associated infertility in assisted reproduction techniques
Endocrine and inflammatory factors and endometriosis-associated infertility in assisted reproduction techniques
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辅助生殖技术中的内分泌和炎症因子以及子宫内膜异位症相关的不孕症
DOI：
10.1007/s00404-012-2567-0
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发表时间：
2013-01-01
影响因子：
2.6
通讯作者：
Wang, Jian
中科院分区：
文献类型：
--
作者：
Du, Yan-Bo;Gao, Min-Zhi;Wang, Jian
本研究旨在探讨子宫内膜异位症对卵泡发育和妊娠的影响，以及卵泡液中炎性因子（IL 1b、PGE2、PGF2 α和TGF β 2）的参与情况。测量卵泡液中炎症因子（IL 1b、PGE 2、PGF 2 α和TGF β 2）和类固醇激素（E2、孕酮、FSH和LH）的浓度以及血清E2和LH浓度。测定颗粒细胞中IL 1b、Ptgs 2、芳香化酶和PPAR γ的mRNA表达。结果：子宫内膜异位症患者的获卵率、中期II期卵母细胞率、卵裂率、有效胚胎率、妊娠率均明显低于对照组。子宫内膜异位症患者血清E2浓度低于对照组。子宫内膜异位症组颗粒细胞芳香化酶水平低于对照组，而卵泡液中PGE2浓度高于对照组。PGE2、PGF2 α、TGF β 2和IL 1b的浓度彼此显著相关。这些结果表明，ART的结果与血清E2浓度相关，子宫内膜异位症的存在对ART的结果产生不利影响。此外，结果支持，在内分泌和炎症因子中，卵泡液中的PGE 2损害卵母细胞的数量和质量。
Our research aimed to evaluate the effect of endometriosis on folliculogenesis and pregnancy, and to assess the involvement of inflammatory factors (IL1b, PGE2, PGF2 alpha, and TGF beta 2) in follicular fluid.A total of 65 follicular fluid aspirates were collected. Concentrations of inflammatory factors (IL1b, PGE2, PGF2 alpha, and TGF beta 2) and steroid hormones (E2, progesterone, FSH, and LH) within follicular fluid as well as serum E2 and LH concentrations were measured. The mRNA expression of IL1b, Ptgs2, aromatase, and PPAR gamma in granulosa cells was determined. The outcome of ART was monitored and recorded.The oocyte retrieval, rate of metaphase II oocyte, cleavage rate, effective embryo rate, and pregnancy rates of patients with endometriosis were all significantly lower than those of the control patients. In those with endometriosis, serum E2 concentrations were lower than those observed in controls. Aromatase levels in the granulosa cells of the endometriosis group were lower while concentrations of PGE2 in follicular fluid were higher than in the control group. Concentrations of PGE2, PGF2 alpha, TGF beta 2, and IL1b were significantly correlated with each other.These results suggest that the outcomes of ART, in relation to serum E2 concentration, were adversely affected by the presence of endometriosis. Furthermore, the results supported that, among the endocrine and inflammatory factors, PGE2 within the follicular fluid impairs the number and quality of oocytes.