Stable properties of spontaneous EPSCs and miniature retinal EPSCs during the development of ON/OFF sublamination in the ferret lateral geniculate nucleus
Stable properties of spontaneous EPSCs and miniature retinal EPSCs during the development of ON/OFF sublamination in the ferret lateral geniculate nucleus
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DOI：
10.1523/jneurosci.19-01-00236.1999
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发表时间：
1999-01-01
影响因子：
5.3
通讯作者：
Sur, W
中科院分区：
文献类型：
--
作者：
Hohnke, CD;Sur, W
视网膜投射到外侧膝状体核（LGN）在雪貂逐步分离到眼睛特定的板层，随后进入sublaminae，接收输入从ON-中心或OFF-中心传入。为了研究中枢神经系统中活动依赖性生长和重组期间突触功效的发展，我们记录了LGN细胞在ON/OFF亚层压期间的自发EPSC（sEPSC）。我们还专门通过在锶存在下刺激视束并记录诱发的微型EPSC（emEPSC）来检查视网膜输入。在ON/OFF亚层压开始时记录的sEPSC和emEPSC的上升时间、面积、半宽度和衰减时间以及sEPSC的间隔与完成后记录的没有差异。通常，EPSC区域较小（10-20 fC），但神经元内部和神经元之间差异很大。sEPSC的频率也是相当可变的，范围从0.2到5 Hz。sEPSC相当于在河豚毒素存在下记录的微型EPSC，并且sEPSC和emEPSC都是CNQX敏感的。在室温下记录的sEPSC和在34 ℃下记录的sEPSC之间没有观察到差异，并且锶可以代替钙，对sEPSC形状没有影响。这些数据表明，一个显着的稳定性，至少AM PA介导的突触传递的组件在一段时间内的主要突触重排的LGN。
Retinal projections to the lateral geniculate nucleus (LGN) in ferrets progressively segregate into eye-specific laminae and subsequently into sublaminae that receive inputs from either ON-center or OFF-center afferents. To study the development of synaptic efficacy during a period of activity-dependent growth and reorganization in the CNS, we recorded spontaneous EPSCs (sEPSCs) from cells of the LGN during ON/OFF sublamination. We also examined retinal inputs specifically by stimulating the optic tract in the presence of strontium and recording evoked miniature EPSCs (emEPSCs). The rise times, areas, half-widths, and decay times of sEPSCs and emEPSCs and interevent intervals of sEPSCs recorded at the beginning of ON/OFF sublamination were not different from those recorded after its completion. Typically EPSC areas were smalt (10-20 fC) but varied greatly both within and between neurons. The frequency of sEPSCs was also quite variable, ranging from 0.2 to 5 Hz. sEPSCs were equivalent to miniature EPSCs recorded in the presence of tetrodotoxin, and both sEPSCs and emEPSCs were CNQX-sensitive. No difference was observed between sEPSCs recorded at room temperature and those recorded at 34 degrees C, and strontium could be substituted for calcium with no effect on sEPSC shape. These data argue for a remarkable stability in the components of at least AM PA-mediated synaptic transmission during a period of major synaptic rearrangement in the LGN.