Quantitative profiling of Arabidopsis polar glycerolipids in response to phosphorus starvation.: Roles of Phospholipases Dζ1 and Dζ2 in phosphatidylcholine hydrolysis and digalactosyldiacylglycerol accumulation in phosphorus-starved plants
Quantitative profiling of Arabidopsis polar glycerolipids in response to phosphorus starvation.: Roles of Phospholipases Dζ1 and Dζ2 in phosphatidylcholine hydrolysis and digalactosyldiacylglycerol accumulation in phosphorus-starved plants
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DOI：
10.1104/pp.106.085647
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发表时间：
2006-10-01
期刊：
影响因子：
7.4
通讯作者：
Wang, Xuemin
中科院分区：
文献类型：
--
作者：
Li, Maoyin;Welti, Ruth;Wang, Xuemin
磷是一种必需的常量营养素，通常会限制植物的生长和发育。在磷有限的条件下，植物的膜脂组成发生了很大的变化，以适应磷的缺乏。为了研究植物对磷饥饿反应中脂类物质的变化，并鉴定参与植物对磷饥饿反应的酶类，对拟南芥(Arabiopsis Thaliana)莲座丛和根中140种极性甘油脂的分子谱进行了定量分析，发现磷脂酶D基因敲除突变体PLD Zeta 1、PLD Zeta 2和PLD Zeta 1 pld Zeta 2对磷饥饿的响应是磷脂浓度降低，半乳脂浓度升高。缺磷的拟南芥莲座体中丢失的磷脂被等量的半乳糖脂取代。根中损失的磷脂浓度远大于莲座果。PLD Zeta 1和PLD Zeta 2功能的中断导致磷饥饿根中磷脂酰胆碱和双乳糖二酰甘油的下降幅度较小。结果表明，磷脂酰胆碱在磷饥饿过程中被PLD-Zeta-S降解，为细胞代谢提供无机磷，为半乳糖脂的合成提供甘油二酯。
Phosphorus is an essential macronutrient that often limits plant growth and development. Under phosphorus-limited conditions, plants undergo substantial alterations in membrane lipid composition to cope with phosphorus deficiency. To characterize the changes in lipid species and to identify enzymes involved in plant response to phosphorus starvation, 140 molecular species of polar glycerolipids were quantitatively profiled in rosettes and roots of wild-type Arabidopsis (Arabidopsis thaliana) and phospholipase D knockout mutants pld zeta 1, pld zeta 2, and pld zeta 1pld zeta 2. In response to phosphorus starvation, the concentration of phospholipids was decreased and that of galactolipids was increased. Phospholipid lost in phosphorus-starved Arabidopsis rosettes was replaced by an equal amount of galactolipid. The concentration of phospholipid lost in roots was much greater than in rosettes. Disruption of both PLD zeta 1 and PLD zeta 2 function resulted in a smaller decrease in phosphatidylcholine and a smaller increase in digalactosyldiacylglycerol in phosphorus-starved roots. The results suggest that hydrolysis of phosphatidylcholine by PLD zeta s during phosphorus starvation contributes to the supply of inorganic phosphorus for cell metabolism and diacylglycerol moieties for galactolipid synthesis.