Non-galloylated and galloylated proanthocyanidin oligomers in grape seeds from Vitus vinifera L. cv. Graciano, Tempranillo and Cabernet Sauvignon
Non-galloylated and galloylated proanthocyanidin oligomers in grape seeds from Vitus vinifera L. cv. Graciano, Tempranillo and Cabernet Sauvignon
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DOI：
10.1002/jsfa.2438
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发表时间：
2006-04-30
影响因子：
4.1
通讯作者：
Mitchell, AE
中科院分区：
文献类型：
--
作者：
Núñez, V;Gómez-Cordovés, C;Mitchell, AE
葡萄种子中的非没食子酰化和没食子酰化黄烷-3-醇组合物。采用正相 HPLC 结合电喷雾电离质谱 (LC/ESI-MS) 检测对 2000 年、2001 年和 2002 年在同一地理区域收获的 Graciano、Tempranillo 和 Cabernet Sauvignon 葡萄进行了测定。所有葡萄籽中均鉴定出非没食子酰化和单没食子酰化黄烷 3-醇，最多可达八聚体，二没食子酰化黄烷-3-醇，最多可达七聚体。对三个不同年份收获的三个葡萄品种的 3-黄烷醇组成进行比较表明，随着聚合度的增加，非没食子酰化黄烷-3-醇的水平降低，而单没食子酰化二聚体在所有品种和年份中的含量最高。其他单没食子酰化黄烷-3-醇的含量在不同年份有所不同。丹魄含有最低水平的非没食子酰化和单没食子酰化黄烷 3-醇，而 Graciano 含有最高水平，除了 2001 年份的非没食子酰化黄烷 3-醇，以及 2000 和 2002 年份的非没食子酰化单体。2000 年份的葡萄籽含有最高水平的非没食子酰化和没食子酰化结构。统计分析表明，flavan-3-ols的分布主要由遗传因素决定，并且还受到气候条件的强烈影响。 (D 2006 化学工业学会
Non-galloylated and galloylated flavan-3-ol composition in seeds from Vitis vinifera L. var. Graciano, Tempranillo and Cabernet Sauvignon grapes harvested in 2000, 2001 and 2002 at the same geographical area were determined using normal-phase HPLC coupled with electrospray ionization mass spectrometry (LC/ESI-MS) detection. Non-galloylated and monogalloylated flavan-3-ols up to octamers, and di-, and trigalloylated flavan-3-ols up to heptamers were identified in all grape seeds. Comparisons of the flavan-3-ol composition in three grape varieties harvested in three different years indicate that levels of non-galloylated flavan-3-ols decrease as the degree of polymerization increased, whereas the monogalloylated dimers were present in the highest levels in all varieties and vintages. The levels of other monogalloylated flavan-3-ols varied in different vintages. Tempranillo contained the lowest levels of non-galloylated and monogalloylated flavan-3-ols, whereas Graciano contained the highest levels, with the exception of non-galloylated flavan-3-ols in vintage 2001, and non-galloylated monomers in vintages 2000 and 2002. Grape seeds from vintage 2000 contained the highest levels of both non-galloylated and galloylated structures. Statistical analyses indicate that the distribution of the flavan-3-ols is primarily determined by genetic factors and is also strongly influenced by climate conditions. (D 2006 Society of Chemical Industry