Decrease of Fertilizing Ability of Mouse Spermatozoa after Freezing and Thawing Is Related to Cellular Injury1
Decrease of Fertilizing Ability of Mouse Spermatozoa after Freezing and Thawing Is Related to Cellular Injury1
复制标题
小鼠精子冻融后受精能力下降与细胞损伤有关1
作者：
H. Nishizono;M. Shioda;T. Takeo;T. Irie;N. Nakagata
摘要小鼠精子冷冻保存后的复育性一般低于新鲜精子。这种能力在用于生产转基因小鼠的主要品系C57 BL/6中尤其低。为了解决这一问题，本研究探讨了小鼠精子冷冻后细胞损伤与精子活力的关系。精子活力分析显示，冻存后C57 BL/6 J、BALB/cA和DBA/2N精子的活力差异无统计学意义（分别为67.6%、43.4%和60.0%）。然而，体外受精（IVF）、扫描电镜（SEM）和透射电镜（TEM）的结果显示，异常精子（FAS）的频率与受精率之间存在很强的相关性（FR; C57 BL/6 J：FAS，83.7%; FR，17.0%; BALB/cA：FAS，67.2%; FR，24.2%;和DBA/2N：FAS，10.2%; FR，93.6%），并且对精子的损伤特别定位于头部的顶体和线粒体。
Abstract In general, the fertilizing ability of cryopreserved mouse spermatozoa is less than that of fresh spermatozoa. This ability is especially low in C57BL/6, the main strain used for the production of transgenic mice. To solve this problem, the relationship between cell damage and fertilizing ability in cryopreserved mouse spermatozoa was examined in this study. Sperm motility analysis revealed no significant difference among the motilities of cryopreserved C57BL/6J, BALB/cA, and DBA/2N sperm (67.6%, 43.4%, and 60.0%, respectively) after thawing. However, the results of in vitro fertilization (IVF), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) showed a strong correlation between the frequency of aberrant spermatozoa (FAS) and fertilization rates (FR; C57BL/6J: FAS, 83.7%; FR, 17.0%; BALB/cA: FAS, 67.2%; FR, 24.2%; and DBA/2N: FAS, 10.2%; FR, 93.6%), and damage to spermatozoa was localized particularly in the acrosome of the head and mitochondria.
冷冻保存在含有全卵的培养基中的小鼠精子的受精潜力。
DOI：
10.1006/cryo.1999.2219
发表时间：
2000
期刊：
Cryobiology.
影响因子：
--
作者：
Dewit,M;Marley,WS;Graham,JK
通讯作者：
Graham,JK
DOI：
10.1530/jrf.0.1120011
发表时间：
1998-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
Wakayama, T;Whittingham, DG;Yanagimachi, R
通讯作者：
Yanagimachi, R