Expression of C-type lectin receptors by subsets of dendritic cells in human skin
Expression of C-type lectin receptors by subsets of dendritic cells in human skin
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DOI：
10.1093/intimm/dxh088
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发表时间：
2004-06-01
影响因子：
4.4
通讯作者：
Romani, N
中科院分区：
文献类型：
--
作者：
Ebner, S;Ehammer, Z;Romani, N
C型凝集素是细胞表面受体，其识别碳水化合物结构，所述碳水化合物结构通常是微生物病原体的一部分。这些分子中的几种在树突状细胞上表达并参与抗原摄取。迄今为止，C型凝集素在人皮肤的树突细胞（即表皮的朗格汉斯细胞和真皮树突细胞）上的表达尚未完全研究。因此，我们研究了C型凝集素在原位和树突状细胞通过免疫荧光和流式细胞术从皮肤外植体迁移获得。重点放在DEC-205/CD 205和BDCA-2（浆细胞样树突状细胞的标志物）的表达模式上。原位朗格汉斯细胞表达低水平的DEC-205。在皮肤外植体器官培养中成熟后表达上调。发现大多数真皮树突状细胞对DEC-205和DC-SIGN/CD 209呈阳性。在大多数皮肤样品中发现很少的BDCA-2表达细胞。它们以小群形式位于基底膜下方的真皮中。正常人皮肤中所有类型的树突状细胞中的绝大多数是不成熟表型，即不表达DC-LAMP/CD 208。它的结论是，正常出现的人皮肤窝藏不同的树突状细胞亚群，包括一些分散的BDCA-2表达细胞，大概浆细胞样树突状细胞，表达可变集的C型凝集素受体。这可能对皮肤免疫系统灵活应对微生物病原体世界的能力做出重要贡献。
C-type lectins are cell surface receptors that recognize carbohydrate structures which are often part of microbial pathogens. Several of these molecules are expressed on dendritic cells and are involved in antigen uptake. Expression of C-type lectins on dendritic cells of the human skin, i.e. Langerhans cells of the epidermis and dermal dendritic cells, has been incompletely studied to date. We therefore investigated C-type lectins in situ and on dendritic cells obtained by migration from skin explants by immunofluorescence and flow cytometry. Emphasis was laid on expression patterns of DEC-205/CD205 and BDCA-2, a marker for plasmacytoid dendritic cells. Langerhans cells in situ expressed low levels of DEC-205. Expression was upregulated upon maturation in skin explant organ culture. Most dermal dendritic cells were found to be positive for DEC-205 and DC-SIGN/CD209. Few BDCA-2-expressing cells were found in most skin samples. They were located in small groups in the dermis close beneath the basement membrane. The vast majority of all types of dendritic cells in normal human skin was of immature phenotype, i.e. did not express DC-LAMP/CD208. It is concluded that normal appearing human skin harbors different subsets of dendritic cells including few scattered BDCA-2-expressing cells, presumably plasmacytoid dendritic cells, expressing variable sets of C-type lectin receptors. This may critically contribute to the capacity of the skin immune system to flexibly respond to the world of microbial pathogens.