THE ORGANIZATION OF THE HUMAN MYELIN BASIC-PROTEIN GENE - COMPARISON WITH THE MOUSE GENE
THE ORGANIZATION OF THE HUMAN MYELIN BASIC-PROTEIN GENE - COMPARISON WITH THE MOUSE GENE
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DOI：
10.1515/bchm3.1989.370.1.503
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发表时间：
1989-05-01
期刊：
影响因子：
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通讯作者：
STOFFEL, W
中科院分区：
文献类型：
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作者：
STREICHER, R;STOFFEL, W
人类髓鞘碱性蛋白基因的组织结构已经建立。该基因由7个外显子组成，分布在32-34 kb之间。测定了7个外显子、5“非编码区的延伸部分以及与外显子相邻的上下游区域的核苷酸序列。对人MBP基因的核苷酸序列与小鼠MBP cDNA的核苷酸序列及小鼠MBP的部分基因结构进行了比较。该基因的7个外显子及5“调控区和部分3”非编码区具有高度的保守性：人和小鼠基因编码区的核苷酸序列显示出93%的同源性。就已报道的478 bp的5“-非编码区和上游调控区而言，这些区域具有80%以上的同源性。引物延伸实验将转录起始位点定位在-55、-82和-183处。在MBP基因的5“调控区发现三个直接重复序列，一个位于-401/-416的九聚体和两个位于-192/-216和-492/-508的八聚体。十聚体序列（从翻译起始的-256至-265）与人PLP调节5“-非编码区的序列完全同源。
The organization of the gene of the human myelin basic protein has been established. The gene contains seven exons distributed over 32-34 kb. The nucleotide sequences of the seven exons, extensive parts at the 5'' noncoding region and upstream and downstream regions adjacent to the exons were sequenced. A comparison of the nucleotide of the human gene with mouse MBP cDNA and parts of the gene structure of mouse MBP has been carried out. There is a high degree of conservation in the seven exons and the 5''-regulatory and part of the 3''-noncoding region: the nucleotide sequence of the coding region of the human and mouse gene show a 93% homology. As far as the reported 5''-noncoding and upstream regulatory regions of 478 bp are comparable these regions are more than 80% homologous. Primer extension experiments positioned the transcription initiation sites at -55, -82 and -183. In the 5''-regulatory region of MBP gene three direct repeats were found, a nonameric at -401/-416 and two octameric at -192/-216 and -492/-508. A decameric sequence (-256 to -265 from the translation start) is completely homologous to a sequence of the human PLP regulatory 5''-noncoding region.