Increased production of natural moisturizing factors and bleomycin hydrolase activity in elderly human skin.
Increased production of natural moisturizing factors and bleomycin hydrolase activity in elderly human skin.
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增加老年人皮肤天然保湿因子的产生和博莱霉素水解酶活性。
DOI：
10.1016/j.jdermsci.2023.03.001
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Takamori K.
中科院分区：
文献类型：
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作者：
Tsurumachi M;Kamata Y;Tominaga M;Ishikawa J;Hideshima T;Shimizu E;Kaneko T;Suga Y;Takamori K.
背景资料：博来霉素水解酶（BH）在颗粒层和下角质层（SC）中表达，参与最终的聚丝蛋白降解。此外，BH在产生游离氨基酸方面起着至关重要的作用，这些氨基酸构成了大多数天然保湿因子（NMF）。然而，BH表达和蛋白酶活性对人类皮肤衰老的影响仍不清楚。目的：本研究旨在评估BH的活性和表达模式的SC提取物从健康的年轻人和老年人individuals.Methods：SC样品通过胶带剥离收集。通过测量瓜氨酸氨肽酶活性来评估BH活性。BH表达通过蛋白质印迹法测定，NMF通过液相色谱/质谱法定量。皮肤屏障功能测定通过测量SC水合作用，经表皮水分损失（TEWL），和皮肤pH值。结果：BH的活性和表达在老年皮肤高于年轻皮肤，BH活性与BH表达水平相关。NMF评价显示，老年皮肤中总氨基酸（如甘氨酸、丝氨酸、天冬氨酸、瓜氨酸、吡咯烷酮羧酸（谷氨酸的代谢物）和反式尿刊酸（组氨酸的代谢物））的水平显著高于年轻皮肤。此外，老年人的SC水合作用和TEWL显著较低，表明皮肤干燥，老年人的pH值显著较高，表明皮肤碱化程度较高。结论：这些结果表明，BH活性和表达以及NMF氨基酸在老年人中增加，作为对抗皮肤干燥的补偿机制。© 2023由Elsevier BV代表日本皮肤病研究学会出版。
Background: Bleomycin hydrolase (BH), which is expressed in the stratum granulosum and lower stratum corneum (SC), is involved in final filaggrin degradation. Furthermore, BH plays an essential role in producing free amino acids, which constitute the majority of natural moisturizing factors (NMF). However, the effects of BH expression and protease activity on human skin aging remain unclear. Objective: This study was designed to evaluate the activity and expression patterns of BH in SC extracts from healthy young and elderly individuals.Methods: SC samples were collected by tape stripping. BH activity was assessed by measuring the citrulline aminopeptidase activity. BH expression was determined by Western blotting, and NMF was quantified by liquid chromatography/mass spectrometry. Skin barrier function was determined by measuring SC hydration, transepidermal water loss (TEWL), and skin pH. Results: The activity and expression of BH were higher in the elderly skin than in young skin, and BH activity was correlated with BH expression levels. Evaluation of the NMF showed that the levels of total amino acids, such as glycine, serine, aspartic acid, citrulline, pyrrolidone carboxylic acid (a metabolite of glutamic acid), and trans-urocanic acid (a metabolite of histidine), were significantly higher in elderly skin than in young skin. Moreover, SC hydration and TEWL were significantly lower in elderly, indicating dry skin, and pH was significantly higher in elderly, indicating greater skin alkalinization. Conclusion: These results suggest that BH activity and expression, as well as NMF amino acids, increase in elderly people as compensatory mechanisms against dry skin.© 2023 Published by Elsevier BV on behalf of Japanese Society for Investigative Dermatology.