Serum-starvation-induced changes in protein synthesis and morphology of Borrelia burgdorferi
Serum-starvation-induced changes in protein synthesis and morphology of Borrelia burgdorferi
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DOI：
10.1099/00221287-146-1-119
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发表时间：
2000-01-01
期刊：
影响因子：
2.8
通讯作者：
Nelson, DR
中科院分区：
文献类型：
--
作者：
Alban, PS;Johnson, PW;Nelson, DR
先前已经证明，当在缺乏兔血清的BSKII（一种复合培养基）中孵育数周时，活动的伯氏疏螺旋体细胞转化为非活动的囊形式。B。Burgdorferi细胞不能从头合成脂肪酸，而血清被认为是脂肪酸和脂质的来源。当B. Burgdorferi细胞在限定的RPMI培养基中是血清饥饿的，类似于90%的细胞在48小时内形成球形囊。四环素可抑制囊肿形成。通过加入BSKII或兔血清，可迅速诱导囊肿开放和营养细胞恢复。从包囊中回收的活细胞百分比范围为2.9%至52.5%，活力与包囊年龄成反比。由B合成蛋白质。用Tran（35）S-Label标记细胞，用双向凝胶电泳和荧光分析法检测血清饥饿对Burgdorferi的影响。血清饥饿诱导了20多种蛋白质的合成。用来自任一B的血清探测来自营养细胞和包囊的蛋白质的蛋白质印迹。感染伯氏菌的人或猴显示几种囊蛋白是抗原性的。提示B.虽然Burgdorferi的基因组很小，生物合成能力非常有限，但它通过诱导蛋白质合成和细胞形态的变化对血清饥饿的条件迅速作出反应。这项研究可能有助于解释B细胞如何在血清饥饿条件下产生蛋白质合成和细胞形态的变化。伯氏疏螺旋体可以在不同的宿主和宿主组织中的营养缺乏时期存活。
It has been demonstrated previously that motile Borrelia burgdorferi cells transform into non-motile cyst-forms when incubated for several weeks in BSKII (a complex medium) lacking rabbit serum. B. burgdorferi cells cannot synthesize fatty acids de novo and serum is thought to provide a source of fatty acids and lipids. When B. burgdorferi cells were serum-starved in defined RPMI medium, similar to 90% of the cells formed spherical cysts within 48 h. Cyst formation was inhibited by tetracycline. Cyst opening and recovery of vegetative cells was rapidly induced by the addition of either BSKII or rabbit serum. The percentage of viable cells recovered from cysts ranged from 2.9 % to 52.5 %, Viability was inversely proportional to cyst age. Protein synthesis by B. burgdorferi during serum starvation was examined by labelling cells with Tran(35)S-Label and analysing the labelled proteins by two-dimensional gel electrophoresis and fluorography. The synthesis of over 20 proteins was induced during serum starvation. Western blots of proteins from vegetative cells and cysts probed with sera from either B. burgdorferi-infected humans or monkeys revealed that several cyst proteins were antigenic. These data suggest that cells of B. burgdorferi, although possessing a small genome and extremely limited biosynthetic capabilities, rapidly respond to conditions of serum starvation by inducing changes in protein synthesis and cell morphology, This study may help explain how cells of B. burgdorferi can survive periods of nutrient deprivation in different hosts and host tissues.