Duplication and parallel evolution of the pancreatic ribonuclease gene (RNASE1) in folivorous non-colobine primates, the howler monkeys (Alouatta spp.)
Duplication and parallel evolution of the pancreatic ribonuclease gene (RNASE1) in folivorous non-colobine primates, the howler monkeys (Alouatta spp.)
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DOI：
10.1038/s41598-019-56941-7
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发表时间：
2019-12
影响因子：
4.6
通讯作者：
Mareike C. Janiak;Andrew S. Burrell;Joseph D. Orkin;T. Disotell
中科院分区：
文献类型：
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作者：
Mareike C. Janiak;Andrew S. Burrell;Joseph D. Orkin;T. Disotell
在前肠发酵的哺乳动物（例如疣猴、偶蹄类反刍动物）中，最初参与免疫防御的胰核糖核酸酶 (RNA酶1) 和溶菌酶 C (LYZ) 已进化出新的消化功能。吼猴是食叶性非疣猴灵长类动物，缺乏疣猴的多室胃，而是通过盲肠结肠区域的发酵来消化叶子。我们提供了四种吼猴 (Alouattaspp.) 的 RNASE1 和 LYZ 基因的数据。我们发现吼猴LYZ是保守的，并且不具有在疣猴和牛序列中发现的取代，而RNA酶1在A的共同祖先中重复。帕利亚塔，A.塞尼古鲁斯，A.萨拉，A.皮格拉。虽然亲本基因 (RNASE1) 是保守的，但子基因 (RNASE1B) 具有多个氨基酸取代，这些取代与疣猴 RNASE1B 基因中发现的氨基酸取代相似。 Alouatta 中重复的 RNase 具有与疣猴相似的生化变化，表明可能具有新的消化功能。这些发现表明，胰腺核糖核酸酶同时在前肠和后肠发酵的叶食灵长类动物中进化出消化微生物发酵产物的新作用。这可能是一种重要的消化酶适应，使吼猴能够在水果供应不足的时期靠树叶生存。
In foregut-fermenting mammals (e.g., colobine monkeys, artiodactyl ruminants) the enzymes pancreatic ribonuclease (RNASE1) and lysozyme C (LYZ), originally involved in immune defense, have evolved new digestive functions. Howler monkeys are folivorous non-colobine primates that lack the multi-chambered stomachs of colobines and instead digest leaves using fermentation in the caeco-colic region. We present data on theRNASE1andLYZgenes of four species of howler monkey (Alouattaspp.). We find that howler monkeyLYZis conserved and does not share the substitutions found in colobine and cow sequences, whereasRNASE1was duplicated in the common ancestor ofA. palliata,A. seniculus,A. sara, andA. pigra. While the parent gene (RNASE1) is conserved, the daughter gene (RNASE1B) has multiple amino acid substitutions that are parallel to those found inRNASE1Bgenes of colobines. The duplicated RNase inAlouattahas biochemical changes similar to those in colobines, suggesting a novel, possibly digestive function. These findings suggest that pancreatic ribonuclease has, in parallel, evolved a new role for digesting the products of microbial fermentation in both foregut- and hindgut-fermenting folivorous primates. This may be a vital digestive enzyme adaptation allowing howler monkeys to survive on leaves during periods of low fruit availability.