A tissue culture assay of corneal epithelial wound closure.
A tissue culture assay of corneal epithelial wound closure.
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角膜上皮伤口闭合的组织培养测定。
DOI：
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发表时间：
1986
影响因子：
4.4
通讯作者：
A. Neufeld
中科院分区：
文献类型：
--
作者：
M. Jumblatt;A. Neufeld
实验测定已开发使用培养的组织来源于兔角膜上皮，研究迁移的上皮片在伤口闭合和细胞基质粘附。为了研究伤口闭合，通过局部冷冻技术在24孔多孔板中体外产生直径为6 mm的上皮缺损，并通过染色随时间定量剩余缺损的大小。为了研究粘附，将培养的细胞用3 H-亮氨酸标记，悬浮，并加入到新鲜的培养板中。在不同时间裂解贴壁细胞，并测定裂解物的放射性。血清可促进实验性缺损的闭合，但层粘连蛋白和纤连蛋白无作用。改变有丝分裂速率的试剂，如表皮生长因子和5-氟尿嘧啶，在本试验中不影响伤口闭合速率。提高细胞内环AMP水平的化合物抑制伤口闭合，但促进细胞-基质粘附。因此，培养的角膜上皮细胞可用于测定对支配浅表上皮伤口闭合的迁移事件的影响。
Experimental assays have been developed using cultured tissue derived from rabbit corneal epithelium to study migration of epithelial sheets during wound closure and cell-substrate adhesion. To study wound closure, epithelial defects, 6 mm in diameter, were produced in vitro in 24 well multiplates by a local freezing technique, and the size of the remaining defect was quantitated over time by staining. To study adhesion, cultured cells were labeled with 3H-leucine, suspended, and added to fresh culture plates. At various times, adherent cells were lysed and the radioactivity of the lysate was determined. Serum enhances the closure of experimental defects, but laminin and fibronectin have no effect. Agents which alter mitotic rate, such as epidermal growth factor and 5-fluorouracil, do not influence the rate of wound closure in this assay. Compounds which elevate intracellular levels of cyclic AMP inhibit wound closure but promote cell-substrate adhesion. Thus, cultured corneal epithelial cells may be used to assay for influences on the migratory events governing closure of superficial epithelial wounds.
影响因子：
2.7
作者：
GIPSON, IK;KIORPES, TC;BRENNAN, SJ
通讯作者：
BRENNAN, SJ