Photo-dependent protein biosynthesis using a caged aminoacyl-tRNA
Photo-dependent protein biosynthesis using a caged aminoacyl-tRNA
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DOI：
10.1016/j.bmcl.2014.10.053
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发表时间：
2014-12-01
影响因子：
2.7
通讯作者：
Ohtsuki, Takashi
中科院分区：
文献类型：
--
作者：
Akahoshi, Akiya;Doi, Yoshio;Ohtsuki, Takashi
Translation systems with four-base codons provide a powerful strategy for protein engineering and protein studies because they enable site-specific incorporation of non-natural amino acids into proteins. In this study, a caged aminoacyl-tRNA with a four-base anticodon was synthesized. The caged aminoacyl-tRNA contains a photocleavable nitroveratryloxycarbonyl (NVOC) group. This study showed that the caged aminoacyl-tRNA was not deacylated, did not bind to EF-Tu, and was activated by light. Photo-dependent translation of an mRNA containing the four-base codon was demonstrated using the caged aminoacyl-tRNA. (C) 2014 Elsevier Ltd. All rights reserved.
