THE PRODUCT OF A FOS-RELATED GENE, FRA-1, BINDS COOPERATIVELY TO THE AP-1 SITE WITH JUN - TRANSCRIPTION FACTOR AP-1 IS COMPRISED OF MULTIPLE PROTEIN COMPLEXES
THE PRODUCT OF A FOS-RELATED GENE, FRA-1, BINDS COOPERATIVELY TO THE AP-1 SITE WITH JUN - TRANSCRIPTION FACTOR AP-1 IS COMPRISED OF MULTIPLE PROTEIN COMPLEXES
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DOI：
10.1101/gad.3.2.173
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发表时间：
1989-02-01
影响因子：
10.5
通讯作者：
CURRAN, T
中科院分区：
文献类型：
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作者：
COHEN, DR;FERREIRA, PCP;CURRAN, T
fra-1 编码与 Fos 抗原相关的血清诱导蛋白 (Fra-1)。我们使用针对 Fra-1 蛋白多个区域的抗体，对血清刺激细胞中的 Fra-1 表达进行了表征。 Fra-1 在 COS 细胞或血清刺激的大鼠成纤维细胞中瞬时表达，主要通过丝氨酸残基的磷酸化进行广泛的翻译后修饰。它存在于细胞核和细胞质中，并参与与 Jun 的蛋白复合物。使用网织红细胞裂解物中合成的蛋白，我们发现 Fra-1 与 Fos 一样，与 Jun 协同结合 AP-1 识别元件。包含亮氨酸拉链区域但不包含相邻碱性氨基酸结构域的截短 Fra-1 蛋白在体外与 Jun 复合，但无法结合 AP-1 寡核苷酸。这些结果表明 Fra-1 有助于转录因子 AP-1 的 DNA 结合活性。
fra-1 encodes a serum-inducible protein (Fra-1) that is antigenically related to Fos. We have characterized Fra-1 expression in serum-stimulated cells using antibodies raised against several regions of this protein. Fra-1, expressed transiently in COS cells or in serum-stimulated rat fibroblasts, undergoes extensive post-translational modification, primarily by phosphorylation of serine residues. It is present in both the nucleus and the cytoplasm and participates in a protein complex with Jun. Using proteins synthesized in reticulocyte lysates, we have shown that Fra-1, like Fos, binds to the AP-1 recognition element cooperatively with Jun. A truncated Fra-1 protein that contains the leucine zipper region but not an adjacent basic amino acid domain, complexes with Jun in vitro but fails to bind AP-1 oligonucleotides. These results demonstrate that Fra-1 contributes to the DNA-binding activity ascribed to transcription factor AP-1.