Mapping of mutations in Pseudomonas aeruginosa defective in pyoverdin production.
Mapping of mutations in Pseudomonas aeruginosa defective in pyoverdin production.
复制标题
脓毒素生产缺陷的铜绿假单胞菌突变图谱。
DOI：
10.1128/jb.167.1.7-11.1986
复制
发表时间：
1986
影响因子：
3.2
通讯作者：
Cox,CD
中科院分区：
文献类型：
--
作者：
Ankenbauer,R;Hanne,LF;Cox,CD
经化学和转座子诱变，分离出12株产pyoverdin缺陷的铜绿假单胞菌PAO突变体，这些突变体无荧光，不能在含有400 μ m邻羟基苯基乙酸的培养基上生长。4个突变体不能产生羟肟酸，6个是羟肟酸阳性，1个对pyoverdin产生温度敏感，另一个在琥珀酸最小培养基上不能合成pyoverdin，但在Casamino Acids培养基上生长时能够合成pyoverdin （Difco实验室，Detroit, Mich）。这些突变被定位在PAO染色体上。所有影响pyoverdin产量的突变都位于65 ~ 70 min之间，位于catA1和mtu-9002之间。
Twelve mutants of Pseudomonas aeruginosa PAO defective in pyoverdin production were isolated (after chemical and transposon mutagenesis) that were nonfluorescent and unable to grow on medium containing 400 microM ethylenediaminedi(o-hydroxyphenylacetic acid). Four mutants were unable to produce hydroxamate, six were hydroxamate positive, one was temperature sensitive for pyoverdin production, and another was unable to synthesize pyoverdin on succinate minimal medium but was capable of synthesizing pyoverdin when grown on Casamino Acids medium (Difco Laboratories, Detroit, Mich.). The mutations were mapped on the PAO chromosome. All the mutations affecting pyoverdin production were located at 65 to 70 min, between catA1 and mtu-9002.