Sequence of class II major histocompatibility complex genes from MRL mice. Conserved amino acids in the I-E beta chains from autoimmune mice.
Sequence of class II major histocompatibility complex genes from MRL mice. Conserved amino acids in the I-E beta chains from autoimmune mice.
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MRL 小鼠的 II 类主要组织相容性复合体基因序列。
DOI：
10.1002/art.1780341111
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发表时间：
1991
影响因子：
--
通讯作者：
Wegrzyn,L
中科院分区：
文献类型：
--
作者：
Schiffenbauer,J;Wegrzyn,L
II 类主要组织相容性复合体分子在正常和异常免疫反应中都至关重要。在小鼠和人类中，这些分子都与多种疾病的发展有关，包括胰岛素依赖性糖尿病和系统性红斑狼疮。 MRL/lprmouse 品系会自发产生狼疮样疾病，其特征包括抗 DNA 抗体、肾小球肾炎和过早死亡。我们评估了这些小鼠的 II 类分子的结构，以研究可能有助于自身免疫的独特序列。利用聚合酶链反应，我们对 MRL/lprand MRL-+/+ 小鼠的 I-A 和 I-E 基因的第二个外显子进行了测序。我们发现，在核苷酸水平上，MRL II类基因与之前测序的“k”单倍型之间存在一些变化，但在蛋白质水平上，它们是相同的。此外，通过比较几株自身免疫小鼠的II类基因序列，我们发现I-Eβ链的第三个高变区存在保守氨基酸，这可能在自身免疫的发生中发挥重要作用。
Class II major histocompatibility complex molecules are critical in both normal and abnormal immune responses. Both in mice and in humans, these molecules have been implicated in the development of various diseases, including insulin‐dependent diabetes mellitus and systemic lupus erythematosus. The MRL/lprmouse strain spontaneously develops a lupus‐like illness with features that include anti‐DNA antibodies, glomerulonephritis, and early death. We evaluated the structure of class II molecules from these mice, to investigate for unique sequences that might contribute to autoimmunity. Utilizing the polymerase chain reaction, we sequenced the second exons from the I‐A and I‐E genes of MRL/lprand MRL‐+/+ mice. We found that at the nucleotide level, there are several changes between MRL class II genes and the previously sequenced “k” haplotype, but at the protein level, they are identical. Furthermore, by comparing the sequences of class II genes from several strains of autoimmune mice, we found that there are conserved amino acids in the third hypervariable region of the I‐Eβ chain which may be important in the development of autoimmunity.