Ovarian control of gonadotropin hormone-releasing hormone pulse generator activity in the rhesus monkey: duration of the associated hypothalamic signal.
Ovarian control of gonadotropin hormone-releasing hormone pulse generator activity in the rhesus monkey: duration of the associated hypothalamic signal.
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恒河猴中促性腺激素释放激素脉冲发生器活性的卵巢控制：相关下丘脑信号的持续时间。
DOI：
10.1159/000126411
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发表时间：
1993
影响因子：
4.1
通讯作者：
Knobil,E
中科院分区：
文献类型：
--
作者：
O'Byrne,KT;Chen,MD;Nishihara,M;Williams,CL;Thalabard,JC;Hotchkiss,J;Knobil,E
恒河猴GnRH脉冲发生器的活性与外周循环中每次LH脉冲之前从中基底下丘脑记录的多单位电活动（MUA）齐射突然增加有关。在长期切除卵巢的动物中，这些MUA的持续时间为10-25分钟，包括一个短暂的初始“超调”，随后是一个平台期，以快速下降到基线活动结束。然而，在完整的猴子中，MUA截击只持续1-3分钟，这一持续时间相当于去卵巢动物的超调时间。此外，与阉割动物相比，正常动物每次MUA射击时神经元活动的最大频率降低。如早期研究所示，给去卵巢的猴子注射雌二醇可使MUA射击持续时间在3-5小时内减少到正常动物的水平。与雌二醇的这种急性效应相反，卵巢切除术后MUA射击持续时间的增加是一个渐进的现象，需要4-6周才能达到长期去卵巢的猴子所观察到的MUA射击持续时间。在每次MUA截击时，观察到最大神经元频率的增加也有类似的缓慢时间过程。卵巢切除术对MUA射射持续时间和射速的长期影响可能是下丘脑重塑的结果，但这种考虑必须通过雌二醇在数小时内逆转这些现象的观察来缓和。
The activity of the GnRH pulse generator in the rhesus monkey is associated with abrupt increases in multiunit electrical activity (MUA) volleys recorded from the mediobasal hypothalamus that precede each pulse of LH in the peripheral circulation. In long-term ovariectomized animals the duration of these MUA volleys is 10–25 min and consists of a brief initial ‘overshoot’ followed by a plateau phase that ends in a rapid decline to baseline activity. In intact monkeys, however, the MUA volley lasts only 1–3 min, a duration equivalent to the overshoot in ovariectomized animals. In addition, the maximal frequency of neuronal activity during each MUA volley is reduced in normal animals when compared to castrates. As shown in earlier studies, estradiol given to ovariectomized monkeys causes a reduction in the duration of MUA volleys to that characteristic of intact animals within 3–5 h. In contrast to this acute effect of estradiol, the increase in MUA volley duration following ovariectomy is a gradual phenomenon, 4–6 weeks being required to achieve the MUA volley duration observed in long-term ovariectomized monkeys. A similar slow time course was observed for the increase in maximal neuronal frequency during each MUA volley. This protracted effect of ovariectomy on MUA volley duration and firing rate may be the consequence of hypothalamic remodelling but this consideration must be tempered by the observation that estradiol reverses these phenomena within hours.