Lubricin reduces cartilage--cartilage integration.
Lubricin reduces cartilage--cartilage integration.
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DOI：
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发表时间：
2004
期刊：
影响因子：
1.1
通讯作者：
D. Schaefer;D. Wendt;M. Moretti;M. Jakob;G. Jay;M. Heberer;I. Martin
中科院分区：
文献类型：
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作者：
D. Schaefer;D. Wendt;M. Moretti;M. Jakob;G. Jay;M. Heberer;I. Martin
体内的软骨整合不会发生，使得甚至软骨裂缝也不会愈合。这可能不仅是由于软骨细胞进入伤口的途径有限，也可能是由于外源性因素。在本文中，我们测试的假设，润滑素，润滑蛋白生理上存在于滑液中，降低了综合软骨修复能力。使用同心圆刀片制备牛关节软骨的盘/环复合物，并在每周三次施加250 μ g/ml润滑素的情况下或不施加润滑素的情况下培养6周。培养后，通过光学显微镜评估，两组中圆盘和环之间的接触面积百分比相等。整合界面的粘合强度，推出机械测试评估，是显着和显着低于润滑处理的标本（2.5千帕）比对照组（28.7千帕）。番红-O染色的横截面的组织学观察证实了润滑素处理的复合材料中的整合减少。我们的研究结果表明，滑膜环境，通过提供软骨表面的润滑，损害软骨-软骨整合。
Cartilage integration in vivo does not occur, such that even cartilage fissures do not heal. This could be due not only to the limited access of chondrocytes to the wound, but also to exogenous factors. In this paper, we tested the hypothesis that lubricin, a lubricating protein physiologically present in the synovial fluid, reduces the integrative cartilage repair capacity. Disk/ring composites of bovine articular cartilage were prepared using concentric circular blades and cultured for 6 weeks with or without treatment with 250 microg/ml lubricin applied three times per week. Following culture, the percentage of contact area between the disks and the rings, as assessed by light microscopy, were equal in both groups. The adhesive strength of the integration interface, as assessed by push-out mechanical tests, was markedly and significantly lower in lubricin-treated specimens (2.5 kPa) than in the controls (28.7 kPa). Histological observation of Safranin-O stained cross-sections confirmed the reduced integration in the lubricin treated composites. Our findings suggest that the synovial milieu, by providing lubrication of cartilage surfaces, impairs cartilage--cartilage integration.