9 Properties of the Heat Shock Proteins of Escherichia coli and the Autoregulation of the Heat Shock Response
9 Properties of the Heat Shock Proteins of Escherichia coli and the Autoregulation of the Heat Shock Response
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9 大肠杆菌热激蛋白的特性及热激反应的自身调节
DOI：
10.1101/087969427.26.209
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
D. Ang
中科院分区：
文献类型：
--
作者：
C. Georgopoulos;K. Liberek;M. Żylicz;D. Ang
I.前言噬菌体λ/大肠杆菌相互作用的研究始于25年前，已经鉴定了大量有趣和有用的宿主功能(有关总结，请参阅Friedman等人)。1984年；Georgopoulos等人。1990年)。这些细菌功能不仅对噬菌体λ的生长和发育至关重要，而且对细菌的生长也是必不可少的。在不允许的温度下研究λ特异性和宿主特异性表型的能力有助于阐明这些细菌基因的功能。在许多情况下，这些遗传学研究发现了一系列在λ发育的特定步骤中所需的大肠杆菌基因，强烈表明它们的基因产物协同作用来执行特定的λ发育步骤。这些基因在质粒上的可用性以及其中的大量突变是阐明其基因产物作用的分子机制的起点。这些大肠杆菌功能中研究得最好的，即NusA/NusB转录抗终止系统以及DNAK和GroEL伴侣，都被证明是由密切相互作用的部分组成的超分子机器(Friedman等人。1984年；DAS 1992；Georgopoulos 1992；Georgopoulos and Welch 1993)。就这一卷而言，最有趣的大肠杆菌功能是DNAK伴侣机器，由DNAK(HSP70同源物)、DNAJ(Hsp40同源物)和GRPE热休克蛋白组成，以及GroEL伴侣机器，由GroES(HSP10同源物)和GroEL(Hsp60同源物)组成。
I. INTRODUCTION The studies of bacteriophage λ/ Escherichia coli interactions, initiated more than 25 years ago, have resulted in the identification of a plethora of interesting and useful host functions (for summary, see Friedman et al. 1984; Georgopoulos et al. 1990). These bacterial functions are essential not only for bacteriophage λ growth and development, but for bacterial growth as well. The ability to study both λ-specific and host-specific phenotypes at nonpermissive temperatures was instrumental in the elucidation of the function of these bacterial genes. In many instances, these genetic studies identified a series of E. coli genes that are required for a specific step in λ development, strongly suggesting that their gene products act synergistically to carry out the particular λ developmental step. The availability of these genes on plasmids plus the large number of mutations in them was the starting block for the elucidation of the molecular mechanism of the action of their gene products. The best studied of these E. coli functions, namely, the NusA/NusB transcriptional antitermination system and the DnaK and GroEL chaperones, all turned out to be supramolecular machines made up of intimately interacting parts (Friedman et al. 1984; Das 1992; Georgopoulos 1992; Georgopoulos and Welch 1993). For the purpose of this particular volume, the most interesting E. coli functions are the DnaK chaperone machine, composed of the DnaK (the hsp70 homolog), DnaJ (the hsp40 homolog), and GrpE heat shock proteins, and the GroEL chaperone machine, composed of the GroES (the hsp10 homolog) and GroEL (the hsp60...