Analysis of expressed sequence tags from a NaHCO3-treated alkali-tolerant plant, Chloris virgata
Analysis of expressed sequence tags from a NaHCO3-treated alkali-tolerant plant, Chloris virgata
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DOI：
10.1016/j.plaphy.2010.01.024
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发表时间：
2010-04-01
影响因子：
6.5
通讯作者：
Takano, Tetsuo
中科院分区：
文献类型：
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作者：
Nishiuchi, Shunsaku;Fujihara, Kazumasa;Takano, Tetsuo
虎尾草Chloris virgata Swartz（C. virgata）是我国东北盐碱地区的一种禾本科野生植物。为了研究柳杉的耐性机制，我们构建了柳杉全株cDNA文库。virgata，用100 mM NaHCO 3处理24 h，对3168个随机选择的克隆进行测序。大部分（2590个）表达序列标签（EST）与NCBI数据库中的序列显示出显著的相似性。在2590个基因中，有1893个是独特的。基因本体（Gene Ontology，GO）利用BLAST 2GO对1081条EST进行了Slim注释，发现其中75条基因被注释了GO术语“response to stress”、“response to inabiotic stimulation”和“response to biotic stimulation”，表明这些基因可能在柳枝稷的耐受机制中发挥作用。通过实时RT-PCR进一步检测了先前研究中已知的与非生物胁迫耐受性相关的24个基因，以了解它们的表达如何受到NaHCO 3胁迫的影响。NaHCO 3处理上调了病程相关基因（DC 998527）、Win 1前体基因（DC 998617）、过氧化氢酶基因（DC 999385）、核糖体失活蛋白1（DC 999555）、Na+/H+逆向转运蛋白基因（DC 998043）和双组分调节基因（DC 998236）的表达。(c)2010年Elsevier Masson SAS。All rights reserved.
Chloris virgata Swartz (C. virgata) is a gramineous wild plant that can survive in saline-alkali areas in northeast China. To examine the tolerance mechanisms of C virgata, we constructed a cDNA library from whole plants of C. virgata that had been treated with 100 mM NaHCO3 for 24 h and sequenced 3168 randomly selected clones. Most (2590) of the expressed sequence tags (ESTs) showed significant similarity to sequences in the NCBI database. Of the 2590 genes, 1893 were unique. Gene Ontology (GO) Slim annotations were obtained for 1081 ESTs by BLAST2GO and it was found that 75 genes of them were annotated with GO terms "response to stress", "response to abiotic stimulus", and "response to biotic stimulus", indicating these genes were likely to function in tolerance mechanism of C virgata.In a separate experiment, 24 genes that are known from previous studies to be associated with abiotic stress tolerance were further examined by real-time RT-PCR to see how their expressions were affected by NaHCO3 stress. NaHCO3 treatment up-regulated the expressions of pathogenesis-related gene (DC998527), Win1 precursor gene (DC998617), catalase gene (DC999385), ribosome inactivating protein 1 (DC999555), Na+/H+ antiporter gene (DC998043), and two-component regulator gene (DC998236). (c) 2010 Elsevier Masson SAS. All rights reserved.