Mapping transplanted stem cell migration after a stroke: a serial, in vivo magnetic resonance imaging study
Mapping transplanted stem cell migration after a stroke: a serial, in vivo magnetic resonance imaging study
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DOI：
10.1016/j.neuroimage.2003.08.030
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发表时间：
2004-01-01
期刊：
影响因子：
5.7
通讯作者：
Williams, SCR
中科院分区：
文献类型：
--
作者：
Modo, M;Mellodew, K;Williams, SCR
干细胞向病变部位的优先迁移是干细胞的高度期望的性质，其允许在受损脑中移植物植入部位的灵活性。在单侧中风损伤的大鼠中，移植到对侧半球的神经干细胞迁移到损伤的半球，并在缺血性梗死周围区域聚集。迄今为止，神经干细胞在受损脑中的迁移主要是从快照组织学图像推断的。在这项研究中，我们证明，通过预先标记神经干细胞与双峰造影剂钆-罗丹明葡聚糖[GRID，可检测的磁共振成像（MRI）和荧光显微镜]，transemispherical迁移移植的神经干细胞对侧的中风病变，可以在体内遵循的系列MM和随后的组织学分析证实。我们的研究结果表明，神经干细胞迁移从注射道主要是沿着胼胝体移植后7天内和广泛重新填充周围的病变区域植入后14天。相比之下，移植到假手术对照中的神经干细胞没有显示出注射道外的任何实质性迁移，这表明在中风损伤动物中观察到的跨细胞迁移是由于神经干细胞被损伤部位吸引。在体内跟踪神经干细胞对损伤的迁移反应将大大提高我们对最佳移植策略的理解，以及神经干细胞如何促进神经系统疾病的功能和解剖恢复。(C)2003年爱思唯尔公司All rights reserved.
Preferential migration of stem cells toward the site of a lesion is a highly desirable property of stem cells that allows flexibility in the site of graft implantation in the damaged brain. In rats with unilateral stroke damage, neural stem cells transplanted into the contralateral hemisphere migrate across to the lesioned hemisphere and populate the area around the ischaemic infarct. To date, the migration of neural stem cells in the damaged brain has been mainly inferred from snapshot histological images. In this study, we demonstrate that by pre-labelling neural stem cells with the bimodal contrast agent Gadolinium-Rhodamine Dextran [GRID, detectable by both magnetic resonance imaging (MRI) and fluorescent microscopy], the transhemispheric migration of transplanted neural stem cells contralateral to a stroke lesion can be followed in vivo by serial MM and corroborated by subsequent histological analyses. Our results indicate that neural stem cells migrated from the injection tract mainly along the corpus callosum within 7 days of transplantation and extensively re-populated the peri-lesion area by 14 days following implantation. In contrast, neural stem cells transplanted into sham controls did not show any substantial migration outside of the injection tract, suggesting that the transcellosal migration observed in the stroke-lesioned animals is due to neural stem cells being attracted by the lesion site. In vivo tracking of the migration of neural stem cells responding to damage will greatly enhance our understanding of optimal transplantation strategies as well as how neural stem cells promote functional and anatomical recovery in neurological disorders. (C) 2003 Elsevier Inc. All rights reserved.