Percutaneous optical imaging system to track reporter gene expression from vasculatures in vivo.
Percutaneous optical imaging system to track reporter gene expression from vasculatures in vivo.
复制标题
经皮光学成像系统追踪体内脉管系统的报告基因表达。
DOI：
10.1117/1.2209559
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发表时间：
2006
影响因子：
3.5
通讯作者：
Yang,X
中科院分区：
文献类型：
--
作者：
Kar,S;Kumar,A;Gao,F;Qiu,B;Zhan,X;Yang,X
This study develops a percutaneous optical imaging system for tracking fluorescent reporter gene expression in vasculatures. We build a percutaneous optical imaging system that primarily comprised a, semi-rigid, two-port optical probe. The performance of the optical probe is first testedin vitrowith cell phantoms, and then the feasibility of the percutaneous optical imaging system is validatedin vivoin eight femoral artery segments of two pigs. The green fluorescent protein (GFP) gene is locally delivered into four arterial segments, while saline is delivered to the four contralateral arterial segments as controls. The targeted arteries are localized using color Doppler, and thereafter the optical probe is positioned to the target arterial segments under ultrasound guidance. Optical imaging captures are obtained using different exposure times from. Subsequently, the GFP- and saline-targeted arteries are harvested for fluorescent microscopy confirmation. The percutaneous optical probe is successfully positioned at a distance approximatelyfrom the targets in all eight arteries. Thein-vivoimaging shows higher average signal intensity in GFP-treated arteries than in saline-treated arteries. This study demonstrates the potential using the percutaneous optical imaging system to monitor,in vivo, reporter gene expression from vasculatures.
