Drop-of-sample rheometry of biological fluids by noncontact acoustic tweezing spectroscopy
Drop-of-sample rheometry of biological fluids by noncontact acoustic tweezing spectroscopy
复制标题
非接触式声镊光谱法对生物体液进行滴样流变测量
DOI：
10.1039/d2lc00356b
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发表时间：
2022
期刊：
影响因子：
6.1
通讯作者：
Khismatullin, Damir B.
中科院分区：
文献类型：
--
作者：
Kasireddy, Nithya;Orie, Jeremy C.;Khismatullin, Damir B.
流变学性质的知识，如粘度和弹性，是必要的有效的材料加工和运输以及生物分析。现有的流变仪以大的样品体积操作，并引起样品与容器或装置壁的接触，这不足以用于可用性有限的敏感流体的流变分析。在这项工作中，我们介绍了声学镊子光谱（ATS），一种新的非接触式流变技术，操作与一个单一的4-6 μl的流体样品滴。在ATS中，样品液滴被声学悬浮，然后暴露于调制的声学信号以诱导其受迫振荡。时间依赖性的样品粘度和弹性从所得的液滴响应测量。ATS测量的聚合物溶液（葡聚糖，黄原胶，明胶）同意与以前报道的数据。ATS预测血浆的剪切粘度从凝血开始1.5分钟时的1.5 cP增加到9分钟时的3.35 cP，而其剪切弹性模量在3.5 min和6.5 min之间从可忽略的值增加到10.7 Pa。凝血使全血粘度从5.4 cP增加到20.7 cP，弹性从0.1 Pa增加到19.2 Pa，总之，ATS为生物医学研究以及医疗、制药和化工行业提供了灵敏的小体积流变分析的机会。
Knowledge of rheological properties, such as viscosity and elasticity, is necessary for efficient material processing and transportation as well as biological analysis. Existing rheometers operate with large sample volume and induce sample contact with container or device walls, which are inadequate for rheological analysis of sensitive fluids limited in availability. In this work, we introduce acoustic tweezing spectroscopy (ATS), a novel noncontact rheological technique that operates with a single 4–6 μl drop of fluid sample. In ATS, a sample drop is acoustically levitated and then exposed to a modulated acoustic signal to induce its forced oscillation. The time-dependent sample viscosity and elasticity are measured from the resulting drop response. The ATS measurements of polymeric solutions (dextran, xanthan gum, gelatin) agree well with previously reported data. The ATS predicts that the shear viscosity of blood plasma increases from 1.5 cP at 1.5 min of coagulation onset to 3.35 cP at 9 min, while its shear elastic modulus grows from a negligible value to 10.7 Pa between 3.5 min and 6.5 min. Coagulation increases whole blood viscosity from 5.4 cP to 20.7 cP and elasticity from 0.1 Pa to 19.2 Pa at 15 min. In summary, ATS provides the opportunity for sensitive small-volume rheological analysis in biomedical research and medical, pharmaceutical, and chemical industries.