HLA class I and class II allele distribution in the Bubi population from the island of Bioko (Equatorial Guinea).
HLA class I and class II allele distribution in the Bubi population from the island of Bioko (Equatorial Guinea).
复制标题
比奥科岛（赤道几内亚）布比人群中 HLA I 类和 II 类等位基因的分布。
作者：
R. Pablo;J. García;C. Vilches;M. E. Moreno;L. Sanz;M. Rementería;S. Puente;M. Kreisler
我们确定了出生在比奥科岛（赤道几内亚）的100名布比人的HLA频率分布。采用血清学和PCR-SSP法进行HLA-A、-B、-C分型。PCR-SSOP检测DRB1/3/4/5、DQB1和DQA1等位基因。该人群的HLA等位基因分布与其他班图语人群相似；但值得一提的是，布比族中A30、A32、B44、DRB1*1301的频率高于其他班图族，且DR4的缺失。通过对该群体I类和II类基因的克隆和测序，描述了5个新的等位基因变异：B*4407、Cw*0706、Cw*1801、Cw*1802和DQB1*0612，以及5个验证序列：B*3910、B*5703、B*8101、Cw*1203和Cw*1701。在布比地区发现了新的HLA-C、B单倍型：Cw*08-B*57、Cw*18-B*57、Cw*0302-B*53、Cw*07-B*53和Cw*1601-B*63。最常见的7位点单倍型为：A*30-Cw*17-B*42-DRB1*1102-DRB3*0202-DQA1*05-DQB1 *0301。就遗传距离而言，布比人更接近其他班图人，而不是西非人。
We determined the HLA frequency distribution in a sample of 100 Bubi individuals born on the island of Bioko (Equatorial Guinea). HLA-A, -B and -C typing was performed by serology and PCR-SSP. DRB1/3/4/5, DQB1 and DQA1 alleles were determined by PCR-SSOP. The HLA allele distribution of this population group resembles those found in other Bantu-speaking groups; however, the higher frequency of A30, A32, B44, DRB1*1301 in the Bubi with respect to other Bantu groups and the absence of DR4 deserve special mention. The cloning and sequencing of class I and II genes in this population allowed the description of five new allelic variants: B*4407, Cw*0706, Cw*1801, Cw*1802 and DQB1*0612 and five confirmatory sequences: B*3910, B*5703, B*8101, Cw*1203 and Cw*1701. The following new HLA-C,B haplotypes have been found in Bubi: Cw*08-B*57, Cw*18-B*57, Cw*0302-B*53, Cw*07-B*53 and Cw*1601-B*63. The most frequent seven-locus haplotype is: A*30-Cw*17-B*42-DRB1*1102-DRB3*0202-DQA1*05-DQB1 *0301. In terms of genetic distance, the Bubi are closer to other Bantu groups than to West African populations.