An in vitro characterization study of new near infrared dyes for molecular imaging
An in vitro characterization study of new near infrared dyes for molecular imaging
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DOI：
10.1016/j.ejmech.2009.01.019
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发表时间：
2009-09-01
影响因子：
6.7
通讯作者：
Hilger, Ingrid
中科院分区：
文献类型：
--
作者：
Pauli, Jutta;Vag, Tibor;Hilger, Ingrid
研究了一系列花青标记染料 DY-681、DY-731、DY-751 和 DY-776 的光谱特性、稳定性和细胞毒性，以确定体内荧光成像的新工具，并找到我们最近在针对癌胚抗原 (CEA) 的靶标特异性探针中用作荧光标记的 DY-676 的替代品。该探针能够选择性监测小鼠中表达 CEA 的肿瘤细胞，但仅显示出较低的荧光量子产率，因此灵敏度不是最佳的。与临床批准的吲哚菁绿 (ICG) 相比，所有 DY 染料均显示出增强的荧光量子产率、优异的稳定性和较低的细胞毒性。通过 DY-681 以及远红可激发 DY-731 和 DY-751，我们确定了三种染料，与 DY-676 和 ICG 相比，其性能有所改善。 (C) 2009 Elsevier Masson SAS。版权所有。
The spectroscopic properties, stability, and cytotoxicity of series of cyanine labels, the dyes DY-681, DY-731, DY-751, and DY-776, were studied to identify new tools for in vivo fluorescence imaging and to find substitutes for DY-676 recently used by us as fluorescent label in a target-specific probe directed against carcinoembryonic antigen (CEA). This probe enables the selective monitoring of CEA-expressing tumor cells in mice, yet displays only a low fluorescence quantum yield and thus, a non-optimum sensitivity. All the DY dyes revealed enhanced fluorescence quantum yields, a superior stability, and a lower cytotoxicity in comparison to clinically approved indocyanine green (ICG). With DY-681 and far-red excitable DY-731 and DY-751, we identified three dyes with improved properties compared to DY-676 and ICG. (C) 2009 Elsevier Masson SAS. All rights reserved.