Production and distribution of entactin and GP-2 in M1536-B3 cells.
Production and distribution of entactin and GP-2 in M1536-B3 cells.
复制标题
M1536-B3 细胞中巢蛋白和 GP-2 的产生和分布。
DOI：
10.1016/0014-4827(82)90043-x
复制
发表时间：
1982
影响因子：
3.7
通讯作者：
Chung,AE
中科院分区：
文献类型：
--
作者：
Bender,BL;Carlin,B;Jaffe,R;Temple,T;Chung,AE
内胚层分化的小鼠胚胎癌细胞系M1536-B3在细胞培养中产生基底膜相关糖蛋白entactin和GP-2。免疫学技术结合光学和电子显微镜被用来研究这些分子的细胞内分布和命运。这两种蛋白质定位于粗面内质网池和离散的膜结合小泡。没有证据表明它们存在于高尔基体中。膜结合囊泡似乎与质膜融合，从而将其内容物转移到细胞表面上的离散病灶。这些病灶在细胞与细胞接触时变得明显，并在细胞与细胞接触部位突出。它们最终合并成连续的致密染色的无定形物质的细胞外带。这些条带形成了一个由细胞排列的蜂窝状空间。看来，细胞外基质的产生可能是由细胞-细胞接触刺激的，并且细胞密度而不是细胞培养物的年龄决定了细胞外分布的模式。这些结果支持这一假设，即这些分子参与细胞粘附和多细胞组织。
The endodermally differentiated mouse embryonal carcinoma cell line M1536-B3 produces the basement membrane-associated glycoproteins entactin and GP-2 in cell culture. Immunological techniques coupled with light and electron microscopy were used to study the intracellular distribution and fate of these molecules. The two proteins were localized in cisternae of rough endoplasmic reticulum and in discrete membrane-bound vesicles. There was no evidence for their presence in the Golgi apparatus. The membrane-bound vesicles appeared to fuse with the plasma membrane and thereby transfer their contents to discrete foci on the cell surface. These foci became apparent as cell-to-cell contact was made and were prominent at cell-cell contact sites. They eventually coalesced into continuous densely stained extracellular bands of amorphous material. These bands formed a honeycomb of spaces lined by cells. It appeared that production of the extracellular matrix was probably stimulated by cell-cell contact, and that cell density rather than age of the cell cultures dictated the pattern of extracellular distribution. These results support the hypothesis that these molecules are involved in cell adhesion and multicellular organization.