Life span of circulating blood cells in ginbuna crucian carp (Carassius auratus langsdorfii)
Life span of circulating blood cells in ginbuna crucian carp (Carassius auratus langsdorfii)
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DOI：
10.1006/fsim.1998.0144
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发表时间：
1998-07-01
影响因子：
4.7
通讯作者：
Nakanishi, T
中科院分区：
文献类型：
--
作者：
Fischer, U;Ototake, M;Nakanishi, T
研究了不同种群银鱼外周血细胞的寿命和半衰期。用荧光细胞连接试剂盒对红细胞或外周血白细胞(PBL)进行体外染色，然后重新注入同一条鱼体内。从外周血中重新分离血细胞，并绘制标记细胞水平随时间的下降曲线。外周血中染色细胞呈指数下降，由此计算细胞的半衰期。循环红细胞的半衰期约为51天。然而，少数红细胞在外周血中存活了270天以上。据估计，循环中的血小板寿命约为70天，半衰期不到5天。循环单核细胞半衰期为2~3天，粒细胞半衰期为2~27天，外周血淋巴细胞半衰期为10~23天。然而，一些染色的淋巴细胞在外周血中持续存在超过145天，这些可能代表记忆细胞。(C)1998年学术出版社。
Life span and half-life of different populations of circulating peripheral blood cells from ginbuna crucian carp (Carassius auratus langsdorfii) were investigated. Erythrocytes or peripheral blood leukocytes (PBL) were stained ex vivo with a fluorescent cell linker kit, then reinjected into the same fish. Blood cells were reisolated from peripheral blood and the decrease in levels of labelled cells was plotted against time. The decrease of stained cells in peripheral blood was exponential, from which the half-life of the cells was calculated. Circulating erythrocytes showed a half-life of approximately 51 days. However, a few erythrocytes survived in the peripheral blood for more than 270 days. The life span of circulating thrombocytes was estimated to be approximately 70 days, with a half-life of less than 5 days. The half-life of circulating monocytes and granulocytes was between 2 and 3 days, respectively, with their life span varying from between 2 and 27 days, while the life-span of circulating lymphocytes ranged from between 10 and 23 days. However, some stained lymphocytes persisted in the peripheral blood for more than 145 days and these may represent memory cells. (C) 1998 Academic Press.