Electroporation of cells and tissues
Electroporation of cells and tissues
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DOI：
10.1109/27.842820
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发表时间：
2000-02-01
影响因子：
1.5
通讯作者：
Weaver, JC
中科院分区：
文献类型：
--
作者：
Weaver, JC
假设电脉冲使流体脂质双层膜的跨膜电压至少达到约0.2V，通常为0.5-1V，以产生最小半径类似于1 nm的原生膜“孔”，通过动态孔群传输小离子，如Na+和Cl-，使膜放电，即使在外部脉冲的情况下，U-m也会增加，导致戏剧性的电行为。通过初生孔和经二次加工扩大的孔的分子运输为分子进出生物细胞提供了基础，细胞体外电穿孔主要用于DNA导入的转基因，但也有许多其他干预措施，包括微生物杀灭。体外电穿孔提供了对细胞的操纵，这些细胞被重新引入体内提供治疗。在体内，组织的电穿孔增强了分子通过组织并进入其构成细胞的运输，组织电穿孔通过更长、更大的脉冲参与了电击损伤。通过更短、更小的脉冲进行组织电穿孔正在研究中，以用于癌症治疗、基因治疗和经皮给药的医学治疗的生物医学工程应用。这种破损涉及一种复杂的屏障，它既包含高电阻的多层脂质双层膜，又包含坚韧的、电不可见的蛋白质基质。
Electrical pulses that cause the transmembrane voltage of fluid lipid bilayer membranes to reach at least U-m approximate to 0.2 V, usually 0.5-1 V, are hypothesized to create primary membrane "pores" with a minimum radius of similar to 1 nm, Transport of small ions such as Na+ and Cl- through a dynamic pore population discharges the membrane even white an external pulse tends to increase U-m, leading to dramatic electrical behavior. Molecular transport through primary pores and pores enlarged by secondary processes provides the basis for transporting molecules into and out of biological cells, Cell electroporation in vitro is used mainly for transfection by DNA introduction, but many other interventions are possible, including microbial killing. Ex vivo electroporation provides manipulation of cells that are reintroduced into the body to provide therapy. In vivo electroporation of tissues enhances molecular transport through tissues and into their constituative cells, Tissue electroporation, by longer, large pulses, is involved in electrocution injury. Tissue electroporation by shorter, smaller pulses is under investigation for biomedical engineering applications of medical therapy aimed at cancer treatment, gene therapy, and transdermal drug delivery. The tatter involves a complex barrier containing both high electrical resistance multilamellar lipid bilayer membranes and a tough, electrically invisible protein matrix.