MOST ULTRAVIOLET-IRRADIATION INDUCED MUTATIONS IN THE NEMATODE CAENORHABDITIS-ELEGANS ARE CHROMOSOMAL REARRANGEMENTS
MOST ULTRAVIOLET-IRRADIATION INDUCED MUTATIONS IN THE NEMATODE CAENORHABDITIS-ELEGANS ARE CHROMOSOMAL REARRANGEMENTS
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DOI：
10.1016/0027-5107(91)90131-7
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发表时间：
1991-07-01
期刊：
影响因子：
--
通讯作者：
BAILLIE, DL
中科院分区：
文献类型：
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作者：
STEWART, HI;ROSENBLUTH, RE;BAILLIE, DL
在这项研究中，我们确定了 254 nm 紫外线 (UV) 作为线虫诱变工具的效用。我们已经证明，成年雌雄同体的辐射提供了一种诱导可遗传的染色体重排的简单方法。采用筛选方案来鉴定由易位 eT1(III;V) 平衡的 40 个图谱单元区域中的隐性致死突变或 unc-36(III) 重复。 120 J/m2 剂量后，3% 的筛选染色体恢复了突变。 该速率类似于以类似方式选择的 1500 R 伽马射线诱导突变的速率。这些突变被分类为致死突变（映射到连锁组 (LG)III 或 LGV）或假定的 unc-36 重复。与微生物中分析的大多数紫外线诱导突变相反，我们发现很大一部分线虫紫外线诱导突变不是简单的基因内损伤，而是多个相邻基因的缺陷或更复杂的事件。这一结论的初步证据来自于高频率的突变，这些突变具有显着效应，导致成年后代数量减少。随后，9 个分析的 LGV 突变中有 6 个被发现存在缺陷。还鉴定出的其他特定重排包括：一种易位，sT5(II; III)，以及两种 unc-36 重复，sDp8 和 sDp9。结论是，紫外线照射可以很容易地用作分析线虫染色体的附加工具，并且线虫应该被证明是一种有用的生物体，可用于研究紫外线在复杂真核生物细胞中作为诱变剂的机制。
In this study we have determined the utility of 254-nm ultraviolet light (UV) as a mutagenic tool in C elegans. We have demonstrated that irradiation of adult hermaphrodites provides a simple method for the induction of heritable chromosomal rearrangements. A screening protocol was employed that identifies either recessive lethal mutations in the 40 map unit region balanced by the translocation eT1(III; V), or unc-36(III) duplications. Mutations were recovered in 3% of the chromosomes screened after a dose of 120 J/m2. This rate resembles that for 1500 R gamma-ray-induced mutations selected in a similar manner. The mutations were classified either as lethals [mapping to Linkage Group (LG)III or LGV] or as putative unc-36 duplications. In contrast to the majority of UV-induced mutations analysed in microorganisms, we found that a large fraction of the C elegans UV-induced mutations are not simple intragenic lesions, but are deficiencies for more than one adjacent gene or more complex events. Preliminary evidence for this conclusion came from the high frequency of mutations that had a dominant effect causing reduced numbers of adult progeny. Subsequently 6 out of 9 analysed LGV mutations were found to be deficiencies. Other specific rearrangements also identified were: one translocation, sT5(II; III), and two unc-36 duplications, sDp8 and sDp9. It was concluded that UV irradiation can easily be used as an additional tool for the analysis of C elegans chromosomes, and that C elegans should prove to be a useful organism in which to study the mechanisms whereby UV acts as a mutagen in cells of complex eukaryotes.