Fractalkine as a marker for assessment of severe acute pancreatitis
Fractalkine as a marker for assessment of severe acute pancreatitis
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DOI：
10.1111/j.1751-2980.2012.00580.x
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发表时间：
2012-04-01
影响因子：
3.5
通讯作者：
Yuan, Yao Zong
中科院分区：
文献类型：
--
作者：
Huang, Li Ya;Chen, Ping;Yuan, Yao Zong
目的：研究FKN在重症急性胰腺炎（SAP）发病中的作用。方法：采用酶联免疫吸附法测定SAP大鼠模型（对照组、SAP 6 h、16 h、24 h、48 h）血清FKN水平。采用逆转录聚合酶链反应（RT-PCR）、免疫印迹和免疫组织化学方法检测胰腺组织中FKN mRNA和蛋白的表达。结果：SAP大鼠血清FKN水平明显升高（与对照组比较P < 0.05）。胰腺和肺组织FKN mRNA和蛋白表达均显著升高，16 h达高峰，肾脏FKN mRNA和蛋白表达48 h达高峰。免疫组化显示FKN在胰腺、肺和肾组织中过度表达。结论：FKN参与了SAP的病情发展，可作为SAP病情评估的一个有价值的指标。
OBJECTIVE: The aim of this study was to study the role of fractalkine (FKN) in the development of severe acute pancreatitis (SAP) in animal model. METHODS: Serum FKN levels in rat model (control, SAP6h, 16h, 24h and 48h) were determined by enzyme-linked immunosorbent assay. FKN mRNA and protein levels in the pancreas tissue were measured by reverse transcription polymerase chain reaction (RT-PCR), Western blot and immunohistochemistry. RESULTS: Serum FKN level in the SAP rat increased significantly (P < 0.05 compared with the control group). FKN mRNA and protein levels in pancreas and lung increased significantly and reached the peak at 16 h after the induction of SAP, while those in kidney reached the peak at 48 h. Immunohistochemistry showed the overexpression of FKN in pancreas, lung and kidney tissue. CONCLUSION: FKN involves in the progression of SAP and might be a valuable marker for the assessment of SAP.