Functional characterization of acetylglutamate synthase and phosphoribosylamine-glycine ligase genes in Gibberella zeae
Functional characterization of acetylglutamate synthase and phosphoribosylamine-glycine ligase genes in Gibberella zeae
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DOI：
10.1007/s00294-006-0110-1
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发表时间：
2007-02-01
期刊：
影响因子：
2.5
通讯作者：
Lee, Yin-Won
中科院分区：
文献类型：
--
作者：
Kim, Jung-Eun;Myong, Kilseon;Lee, Yin-Won
玉米赤霉菌（Gibberellazeae）是禾谷镰刀菌（Fusariumgraminearum）的一种重要病原菌，广泛分布于世界各地。本研究中，我们对两株营养缺陷型菌株进行了鉴定，命名为S4 B1279和S4 B3008。限制性内切酶介导的整合（REMI）。这两种突变株表现出多效性的表型变化，包括减少菌丝生长和毒力和废除有性繁殖。REMI突变体的分子分析表明，S4 B1279的营养缺陷型是由于编码乙酰谷氨酸合酶的ARG 2基因突变所致，S4 B3008的营养缺陷型是由于编码磷酸核糖胺-甘氨酸连接酶的ADE 5基因突变所致。随后的基因破坏和互补研究分别证实了ARG 2和ADE 5在G.玉米。我们的研究证明了利用REMI技术研究G.除了提供允许真菌遗传转化的两个新的选择标记之外，还提供了对zeae毒力机制的研究。
Gibberella zeae (anamorph, Fusarium graminearum) is an important pathogen of cereal crops found in many regions of the world. In this study, we have characterized two auxotrophic strains, designated S4B1279 and S4B3008, which were discovered from a collection of insertional mutants of G. zeae generated by restriction enzyme-mediated integration (REMI). Both mutant strains exhibited pleiotropic phenotypic changes that include reduction of mycelial growth and virulence and abolished sexual reproduction. Molecular analysis of the REMI mutants has shown that the auxotrophy of S4B1279 is due to a mutation of the ARG2 gene encoding an acetylglutamate synthase, and the auxotrophy of S4B3008 is due to a mutation of the ADE5 gene encoding a phosphoribosylamine-glycine ligase. Subsequent gene disruption and complementation studies have confirmed the functions for ARG2 and ADE5, respectively, in G. zeae. Our study has demonstrated the feasibility of using the REMI technique in studying G. zeae virulence mechanisms, in addition to providing two new selectable markers allowing genetic transformation of the fungus.