Effects of N-acyl homoserine lactone analogues on Porphyromonas gingivalis biofilm formation
Effects of N-acyl homoserine lactone analogues on Porphyromonas gingivalis biofilm formation
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DOI：
10.1111/j.1600-0765.2009.01228.x
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发表时间：
2010-04-01
影响因子：
3.5
通讯作者：
Ebisu, S.
中科院分区：
文献类型：
--
作者：
Asahi, Y.;Noiri, Y.;Ebisu, S.
背景与目的：口腔生物膜中的革兰氏阴性厌氧杆菌牙龈卟啉单胞菌是牙周病的主要病原菌。各种革兰氏阴性菌的生物膜形成是由依赖于N-酰基高丝氨酸内酯（HSL）的群体感应电路调节的。一些合成的N-酰基HSL类似物作为群体感应抑制剂，抑制铜绿假单胞菌生物膜的形成。开发针对口腔生物膜的化学控制剂是必要的，因为到目前为止，生物膜仅通过机械清创来去除。本研究探讨了N-酰基HSL类似物对牙龈卟啉单胞菌生物膜形成的影响，目的是开发新的药物，抑制口腔biofilm formation.Material和方法：流动细胞模型用于牙龈卟啉单胞菌生物膜形成。用分光光度法对17种合成的N-酰基HSL类似物进行了定量评价。结果：17种化合物中有10种对牙龈卟啉单胞菌生物膜形成有抑制作用。10种类似物中有3种显着减少了生物膜形成细胞（p < 0.05），并且这些生物膜结构的三维形成较差。有没有定量或定性的差异之间的控制和三个类似物treated groups.Conclusion：三个合成的N-酰基HSL类似物抑制牙龈卟啉单胞菌生物膜的形成。我们认为这些类似物影响牙龈卟啉单胞菌生物膜形成的发展阶段。
Background and Objective:The gram-negative anaerobic rod Porphyromonas gingivalis in oral biofilms is a primary etiological agent of periodontal disease. Biofilm formation of various gram-negative bacteria is regulated by a quorum-sensing circuit that relies on N-acyl homoserine lactones (HSLs). Some synthetic N-acyl HSL analogues act as quorum-sensing inhibitors and suppress biofilm formation in Pseudomonas aeruginosa. Development of chemical control agents against oral biofilms is necessary, because until now, biofilms have been removed only by mechanical debridement. The present study investigated the effect of N-acyl HSL analogues on P. gingivalis biofilm formation, with the aim of developing new drugs that inhibit oral biofilm formation.Material and Methods:A flow-cell model was used for P. gingivalis biofilm formation. Seventeen synthetic N-acyl HSL analogues were quantitatively assessed by spectrophotometry. The effects of three antagonistic compounds against P. gingivalis biofilm formation were further examined by confocal laser scanning microscopy, and investigated for primary attachment using spectrophotometry and phase contrast microscopy.Results:Ten out of 17 analogues affected P. gingivalis biofilm formation. Three out of 10 analogues significantly decreased biofilm-forming cells (p < 0.05), and these biofilm structures were less well formed three-dimensionally. There were no quantitative or qualitative differences in cell attachment between the control and the three analogue-treated groups.Conclusion:Three synthetic N-acyl HSL analogues inhibited biofilm formation in P. gingivalis. We suggest that these analogues influence the development stage of P. gingivalis biofilm formation.