Sensory stimulus evokes inhibition rather than excitation in cerebellar Purkinje cells in vivo in mice
Sensory stimulus evokes inhibition rather than excitation in cerebellar Purkinje cells in vivo in mice
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感觉刺激在小鼠体内引起小脑浦肯野细胞的抑制而不是兴奋
DOI：
10.1016/j.neulet.2010.10.018
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发表时间：
2011-01-07
影响因子：
2.5
通讯作者：
Qiu, De-Lai
中科院分区：
文献类型：
--
作者：
Chu, Chun-Ping;Bing, Yan-Hua;Qiu, De-Lai
小脑Purkinje细胞（PC）精确地通过颗粒细胞响应了触觉刺激，但是，感觉诱发的突触输入与小脑皮层中输出尖峰的产生模式之间的相互作用尚不清楚。在这项研究中，我们使用电生理记录和药理学方法来研究小脑PC，以响应氨基甲酸酯 - 麻醉小鼠的同侧晶须垫上的天然刺激。我们发现，在同侧晶须垫上的气动刺激既不引起复杂的尖峰也不是简单的尖峰射击，但确实唤起了强烈的GABA（A）受体介导的小脑Cortex Cortex folium crus crus II中PC的抑制作用。来自分子层和PC层的现场电势记录表明，空气刺激刺激引起了一系列平行纤维齐射的序列，然后是GABA（A）受体介导的抑制作用，该抑制被AMPA受体拮抗剂NBQX完全阻塞。细胞连接的录音表明，气动刺激引起了简单的尖峰射击的暂停，GABA（A）受体拮抗剂消除了停顿，发现PC中的触觉刺激引起的尖峰发射。这些结果表明，晶须垫的自然刺激既不引起复合尖峰，也没有触发简单的尖峰，而是在PC中诱导的抑制作用，这表明中间神经元网络被快速激活，并且参与了控制小鼠Cerebellar Cortex Cortex for crus II中感觉信息处理的传播。 （c）2010年Elsevier Ireland Ltd.保留所有权利。
Cerebellar Purkinje cells (PC) response precisely to tactile stimulus via granule cells, however, the interaction between sensory evoked synaptic input and the resulting pattern of output spikes in cerebellar cortex is unclear. In this study, we used electrophysiological recording and pharmacological methods to investigate the cerebellar PC in response to natural stimulus on ipsilateral whisker pad in urethane-anesthetized mice. We found that air-puff stimulus on ipsilateral whisker pad evoked neither complex spikes nor simple spike firing, but indeed evoked a strong GABA(A) receptor-mediated inhibition in PCs in cerebellar cortex folium Crus II. Field potential recordings from both molecular layer and PC layer showed that air-puff stimulus evoked a sequence of parallel fiber volley followed by a GABA(A) receptor-mediated inhibition, which completely blocked by AMPA receptor antagonist, NBQX. Cell-attached recordings showed that air-puff stimulus evoked a pause of simple spike firing, GABA(A) receptor antagonist abolished the pause, revealed the tactile stimulus-evoked spike firing in PCs. These results indicated that natural stimulus of whisker pad neither evoked complex spikes, nor fired simple spikes, but induced inhibition in PCs, suggesting that the interneuron network are rapid activated and involved in controlling the spread of sensory information processing in mouse cerebellar cortex folium Crus II. (C) 2010 Elsevier Ireland Ltd. All rights reserved.