Mutations causing constitutive invertase synthesis in yeast: genetic interactions with snf mutations.
Mutations causing constitutive invertase synthesis in yeast: genetic interactions with snf mutations.
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DOI：
10.1093/genetics/115.2.247
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发表时间：
1987-02
期刊：
影响因子：
3.3
通讯作者：
L. Neigeborn;M. Carlson
中科院分区：
文献类型：
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作者：
L. Neigeborn;M. Carlson
我们已经选择了210个突变体能够在2-脱氧葡萄糖的存在下生长在蔗糖上。我们确定了三个主要互补组的隐性突变，导致组成型（葡萄糖不敏感）分泌型转化酶的合成。两组包含先前鉴定的HXK 2和REG 1基因的等位基因，第三组被指定为HXK 1（组成型转化酶去阻遏）。通过SUC 2-LEU 2-lacZ融合体的组成型表达判断，SUC 2上游调控区介导了SUC 1对SUC 2表达的影响，其中LEU 2启动子受SUC 2上游序列的控制。一个突变也导致麦芽糖酶的葡萄糖不敏感的表达。先前分离的组成型突变ssn 6对于非常高水平的组成型转化酶表达是上位性的。SNF基因的突变，防止去阻遏的转化酶是上位性的，reg 1和hxk 2，我们以前已经表明，ssn 6与SNF突变有不同的上位性关系。组成型突变tup 1在与snf突变的遗传相互作用方面与ssn 6相似。这些发现表明CID 1、REG 1和HXK 2在功能上不同于SSN 6和TUP 1。
We have selected 210 mutants able to grow on sucrose in the presence of 2-deoxyglucose. We identified recessive mutations in three major complementation groups that cause constitutive (glucose-insensitive) secreted invertase synthesis. Two groups comprise alleles of the previously identified HXK2 and REG1 genes, and the third group was designated cid1 (constitutive invertase derepression). The effect of cid1 on SUC2 expression is mediated by the SUC2 upstream regulatory region, as judged by the constitutive expression of a SUC2-LEU2-lacZ fusion in which the LEU2 promoter is under control of SUC2 upstream sequences. A cid1 mutation also causes glucose-insensitive expression of maltase. The previously isolated constitutive mutation ssn6 is epistatic to cid1, reg1 and hxk2 for very high level constitutive invertase expression. Mutations in SNF genes that prevent derepression of invertase are epistatic to cid1, reg1 and hxk2; we have previously shown that ssn6 has different epistasis relationships with snf mutations. The constitutive mutation tup1 was found to resemble ssn6 in its genetic interactions with snf mutations. These findings suggest that CID1, REG1 and HXK2 are functionally distinct from SSN6 and TUP1.