The expression of secretory leukocyte protease inhibitor (SLPI) in the fallopian tube: SLPI protects the acrosome reaction of sperm from inhibitory effects of elastase.
The expression of secretory leukocyte protease inhibitor (SLPI) in the fallopian tube: SLPI protects the acrosome reaction of sperm from inhibitory effects of elastase.
复制标题
DOI：
10.1093/humrep/17.10.2517
复制
发表时间：
2002-10
影响因子：
6.1
通讯作者：
Y. Ota;K. Shimoya;Qing Zhang;A. Moriyama;R. Chin;Kumiko Tenma;T. Kimura;M. Koyama;C. Azuma;Y. Murata
中科院分区：
文献类型：
--
作者：
Y. Ota;K. Shimoya;Qing Zhang;A. Moriyama;R. Chin;Kumiko Tenma;T. Kimura;M. Koyama;C. Azuma;Y. Murata
分泌性白细胞蛋白酶抑制剂（SLPI）是在包括腮腺分泌物、宫颈粘液、精浆和腹水的各种液体中发现的蛋白质，并且是人白细胞弹性蛋白酶活性的有效抑制剂。本研究的目的是双重的，以评价（i）输卵管中SLPI的存在，和（ii）SLPI对精子顶体反应的影响。方法与结果Western blot分析显示，SLPI蛋白在输卵管峡部、壶腹和漏斗部均呈12 kDa条带。使用抗SLPI多克隆抗体的免疫组织化学显示输卵管上皮细胞染色阳性。RT-PCR证实SLPI基因在输卵管中有表达。为了确定SLPI在输卵管中的功能，检测了SLPI和弹性蛋白酶对精子顶体反应的影响。SLPI以剂量依赖性方式阻止弹性蛋白酶引起的顶体反应的减少。结论输卵管SLPI参与精卵相互作用。
BACKGROUND Secretory leukocyte protease inhibitor (SLPI) is a protein found in various fluids, including parotid secretions, cervical mucus, seminal plasma and ascites, and is a potent inhibitor of human leukocyte elastase activity. The objective of the study was 2-fold, to evaluate (i) the presence of SLPI in the Fallopian tube, and (ii) the effect of SLPI on the acrosome reaction of sperm. METHODS AND RESULTS Western blot analysis revealed that SLPI protein was detected as a 12 kDa band in the isthmus, ampulla and infundibulum of the Fallopian tube. Immunohistochemistry using an anti-SLPI polyclonal antibody revealed positive staining of epithelial cells in the Fallopian tube. RT-PCR demonstrated that SLPI transcripts were expressed in the Fallopian tube. To determine the function of SLPI in the Fallopian tube, the effects of SLPI and elastase on the sperm acrosome reaction were examined. SLPI prevented the reduction of the acrosome reaction by elastase in a dose-dependent manner. CONCLUSION The present findings suggest that SLPI in the Fallopian tube contributes to sperm-oocyte interaction.