Acid-base buffering in organ preservation solutions as a function of temperature: new parameters for comparing buffer capacity and efficiency
Acid-base buffering in organ preservation solutions as a function of temperature: new parameters for comparing buffer capacity and efficiency
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DOI：
10.1016/s0011-2240(02)00104-9
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发表时间：
2002-08-01
期刊：
影响因子：
2.7
通讯作者：
Taylor, MJ
中科院分区：
文献类型：
--
作者：
Baicu, SC;Taylor, MJ
控制酸度和预防细胞内酸中毒被认为是有效器官保存溶液的关键特性。因此，缓冲容量和效率对于比较最佳低温保存的保存液的相对优点非常重要，但这些参数不适用于当今移植中感兴趣的各种器官保存溶液。此外，缓冲容量取决于浓度和pH，使得对于含有多种缓冲物质的复杂溶液来说，缓冲容量不容易预测。本研究使用标准电测方法测量酸解离常数，以确定各种新型和常用低温保存溶液的最大和相对缓冲能力与温度的函数关系。这些测量提供的参考数据表明，常用解决方案之间的比较缓冲容量和效率差异很大。此外，含有两性离子磺酸缓冲液（例如 Hepes）的液体在生理重要性范围内对 α-stat pH 调节具有优异的缓冲作用。 (C) 2002 Elsever Science（美国）。版权所有。
Control of acidity and preventing intracellular acidosis are recognized as critical properties of an effective organ preservation solution. Buffer capacity and efficiency are therefore important for comparing the relative merits of preservation fluids for optimum hypothermic storage, but these parameters are not available for the variety of organ preservation solutions of interest in transplantation today. Moreover, buffer capacity is dependent upon both concentration and pH such that buffer capacity is not easily predicted for a complex solution containing multiple buffer species. Using standard electrometric methods to measure acid dissociation constants, this study was undertaken to determine the maximum and relative buffer capacities of a variety of new and commonly used hypothermic preservation solutions as a function of temperature. The reference data provided by these measurements show that comparative buffer capacity and efficiency vary widely between the commonly used solutions. Moreover, the fluids containing zwitterionic sulfonic acid buffers such as Hepes possess superior buffering for alpha-stat pH regulation in the region of physiological importance. (C) 2002 Elsever Science (USA). All rights reserved.