Orthotopic Esophageal Cancers: Intraesophageal Hyperthermia-enhanced Direct Chemotherapy in Rats
Orthotopic Esophageal Cancers: Intraesophageal Hyperthermia-enhanced Direct Chemotherapy in Rats
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DOI：
10.1148/radiol.2016152281
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发表时间：
2017-01-01
期刊：
影响因子：
19.7
通讯作者：
Yang, Xiaoming
中科院分区：
文献类型：
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作者：
Shi, Yaoping;Zhang, Feng;Yang, Xiaoming
目的：为了确定使用食管内射频（RF）热疗，以提高局部化疗在大鼠模型原位食管鳞状cancer.Materials和方法的可行性：动物协议批准的机构动物护理和使用委员会和机构审查委员会。用荧光素酶慢病毒颗粒转导人食管鳞癌细胞。在每组六只动物的四个研究组中，用（a）磁共振成像加热导丝介导的RF热疗的组合疗法治疗癌细胞、具有皮下癌食管异种移植物的小鼠和具有原位食管癌的裸大鼠（42摄氏度）加上局部化疗（顺铂和5-氟尿嘧啶），（B）单独的化疗，（c）单独的RF热疗，和（d）磷酸盐缓冲盐水。采用生物发光光学成像和经皮超声成像观察2周以上各组肿瘤生物发光信号和肿瘤大小变化，并与随后的组织学结果相关联。结果：与单纯化疗、单纯射频热疗和磷酸盐缓冲液相比，射频热疗联合化疗诱导的细胞增殖率最低（甲瓒的相对吸光度：分别为23.4% +/-7，44.6%+/-7.5，95.8%+/-2，100%; P < .0001），使得相对肿瘤体积最小（0.65mm（3）+/-0.65mm（3））。15，P < .0001）和相对生物发光光学成像光子信号（0. 57 × 10（7）光子/秒/平方毫米+/-0.15，P < .001），以及最小的相对肿瘤体积（0.68 mm（3）+/-0.13，P < .05）和相对光子信号（0.56 × 10（7）光子/秒/平方毫米+/-0.11。结论：食管内射频热疗可增强食管鳞癌化疗的疗效。(C)RSNA，2016
Purpose: To determine the feasibility of using intraesophageal radiofrequency (RF) hyperthermia to enhance local chemotherapy in a rat model with orthotopic esophageal squamous cancers.Materials and Methods: The animal protocol was approved by the institutional animal care and use committee and the institutional review board. Human esophageal squamous cancer cells were transduced with luciferase lentiviral particles. Cancer cells, mice with subcutaneous cancer esophageal xenografts, and nude rats with orthotopic esophageal cancers in four study groups of six animals per group were treated with (a) combination therapy of magnetic resonance imaging heating guidewire-mediated RF hyperthermia (42 degrees C) plus local chemotherapy (cisplatin and 5-fluorouracil), (b) chemotherapy alone, (c) RF hyperthermia alone, and (d) phosphate-buffered saline. Bioluminescent optical imaging and transcutaneous ultrasonographic imaging were used to observe bioluminescence signal and changes in tumor size among the groups over 2 weeks, which were correlated with subsequent histologic results. The nonparametric Mann-Whitney U test was used for comparisons of variables.Results: Compared with chemotherapy alone, RF hyperthermia alone, and phosphate-buffered saline, combination therapy with RF hyperthermia and chemotherapy induced the lowest cell proliferation (relative absorbance of formazan: 23.4% +/- 7, 44.6% +/- 7.5, 95.8% +/- 2, 100%, respectively; P < .0001), rendered the smallest relative tumor volume (0.65 mm(3) +/- 0. 15, P < .0001) and relative bioluminescence optical imaging photon signal (0. 57 x 10(7) photons per second per square millimeter +/- 0.15, P < .001) of mice with esophageal cancer xenografts, as well as the smallest relative tumor volume (0.68 mm(3) +/- 0.13, P < .05) and relative photon signal (0.56 X 10(7) photons per second per square millimeter +/- 0.11. P < .001) of rat orthotopic esophageal cancers.Conclusion: Intraesophageal RF hyperthermia can enhance the effect of chemotherapy on esophageal squamous cell cancers. (C) RSNA, 2016