Circulating CD34-positive cells provide an index of cerebrovascular function
Circulating CD34-positive cells provide an index of cerebrovascular function
复制标题
DOI：
10.1161/01.cir.0000133311.25587.de
复制
发表时间：
2004-06-22
期刊：
影响因子：
37.8
通讯作者：
Naritomi, H
中科院分区：
文献类型：
--
作者：
Taguchi, A;Matsuyama, T;Naritomi, H
背景 - 越来越多的证据表明循环内皮祖细胞（包括外周血中存在的 CD34 和 CD133 阳性细胞群）在维持脉管系统和新血管形成中发挥作用。包括CD34阳性细胞在内的未成熟群体已被证明有助于血管稳态，不仅作为内皮祖细胞池，而且作为缺血位点的生长/血管生成因子的来源。我们假设循环未成熟细胞数量的减少可能会损害这种生理和修复过程，可能导致脑血管功能障碍。方法和结果-分析有动脉粥样硬化血栓性脑缺血事件史的患者中循环未成熟细胞、CD34-、CD133-、CD117-和CD135-阳性细胞的水平，以评估 可能与颈动脉粥样硬化程度和脑梗塞数量有关。循环CD34和CD133阳性细胞的数量与脑梗塞之间存在很强的负相关性。相反，动脉粥样硬化的程度与循环未成熟细胞的数量之间没有相关性。对脑动脉闭塞患者的分析显示，循环 CD34 和 CD133 阳性细胞与慢性灌注不足区域的局部血流量之间存在显着正相关。结论 - 这些结果表明，循环 CD34 和 CD133 阳性细胞可能有助于缺血应激环境下脑循环的维持。我们的数据证明了一种简单而精确的方法可用于量化循环 CD34 阳性细胞，后者提供了脑血管功能的标记。
Background - Increasing evidence points to a role for circulating endothelial progenitor cells, including populations of CD34- and CD133-positive cells present in peripheral blood, in maintenance of the vasculature and neovascularization. Immature populations, including CD34- positive cells, have been shown to contribute to vascular homeostasis, not only as a pool of endothelial progenitor cells but also as a source of growth/angiogenesis factors at ischemic loci. We hypothesized that diminished numbers of circulating immature cells might impair such physiological and reparative processes, potentially contributing to cerebrovascular dysfunction.Methods and Results - The level of circulating immature cells, CD34-, CD133-, CD117-, and CD135-positive cells, in patients with a history of atherothrombotic cerebral ischemic events was analyzed to assess possible correlations with the degree of carotid atherosclerosis and number of cerebral infarctions. There was a strong inverse correlation between numbers of circulating CD34- and CD133- positive cells and cerebral infarction. In contrast, there was no correlation between the degree of atherosclerosis and populations of circulating immature cells. Analysis of patients with cerebral artery occlusion revealed a significant positive correlation between circulating CD34- and CD133- positive cells and regional blood flow in areas of chronic hypoperfusion.Conclusions - These results suggest a possible contribution of circulating CD34- and CD133- positive cells in maintenance of the cerebral circulation in settings of ischemic stress. Our data demonstrate the utility of a simple and precise method to quantify circulating CD34- positive cells, the latter providing a marker of cerebrovascular function.