Preadipocyte number in omental and subcutaneous adipose tissue of obese individuals
Preadipocyte number in omental and subcutaneous adipose tissue of obese individuals
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DOI：
10.1038/oby.2004.55
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发表时间：
2004-03-01
期刊：
影响因子：
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通讯作者：
Buurman, WA
中科院分区：
文献类型：
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作者：
Bakker, AHF;van Dielen, FMH;Buurman, WA
目的：确定前脂肪细胞分离程序的变化，并评估肥胖个体皮下和网膜脂肪组织中前脂肪细胞的数量和功能。研究方法和程序：27名BMI为44 +/- 10 kg/m的肥胖受试者腹部皮下和腹部网膜脂肪储存中每克脂肪组织的前脂肪细胞数量（2）结果：前脂肪细胞数的评估被认为是劳动密集型和容易出错。我们的数据表明，间质血管细胞（SVC），从脂肪组织分离的胶原酶消化，是依赖于胶原酶治疗的持续时间和大小和活检的起源。此外，分化的SVC的脂肪积累和瘦素生产依赖于粘附的SVC（aSVC）在培养板中的数量和存在的蛋白质来自血清和过氧化物酶体增殖物激活受体ligands.Discussion：使用我们的标准化的分离和分化协议，我们发现，SVCs，aSVCs，瘦素生产和脂肪积累的数量仍然有很大的个体之间的差异。有趣的是，在个体内，SVCs、aSVCs的数量以及通过区分aSVCs与皮下和网膜脂肪库而产生的瘦素是相关的，而脂肪积累则不是。在肥胖至严重肥胖的受试者中，BMI和年龄的差异不能解释SVC、aSVC、瘦素产生和脂肪积累的差异。
Objective: To determine the variation in preadipocyte isolation procedure and to assess the number and function of preadipocytes from subcutaneous and omental adipose tissue of obese individuals.Research Methods and Procedures: The preadipocyte number per gram of adipose tissue in the abdominal-subcutaneous and abdominal-omental adipose stores of 27 obese subjects with a BMI of 44 +/- 10 kg/m(2) and an age of 40 9 years was determined.Results: The assessment of the preadipocyte number was found to be labor intensive and error prone. Our data indicated that the number of stromal vascular cells (SVCs), isolated from the adipose tissue by collagenase digestion, was dependent on the duration of collagenase treatment and the size and the origin of the biopsy. In addition, the fat accumulation and leptin production by differentiated SVCs were dependent on the number of adherent SVCs (aSVCs) in the culture plate and the presence of proteins derived from serum and peroxisome proliferator-activated receptor ligands.Discussion: Using our standardized isolation and differentiation protocol, we found that the number of SVCs, aSVCs, leptin production, and fat accumulation still varied considerably among individuals. Interestingly, within individuals, the number of SVCs, aSVCs, and the leptin production by differentiating aSVCs from both the subcutaneous and the omental fat depots were associated, whereas fat accumulation was not. In obese to severely obese subjects, differences in BMI and age could not explain differences in SVCs, aSVCs, leptin production, and fat accumulation.